RegulonDB

Gene
Name:	hypD
Synonym(s):	ECK2724, EG10486, b2729, hydF
Genome position(nucleotides):	2852136 --> 2853257
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.44
External database links:

ASAP:	ABE-0008965
CGSC:	33116
ECHOBASE:	EB0481
ECOLIHUB:	hypD
OU-MICROARRAY:	b2729
STRING:	511145.b2729
COLOMBOS:	hypD

Shine dalgarno
Sequence:	gttgtatggcGAGGAAaaataATG

Product

Name:

Fe-(CN)₂CO cofactor assembly scaffold protein HypD

Synonym(s):

HydF, HypD, HypD, scaffold protein for assembly of the Fe-(CN)₂CO cofactor

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

41.363

Isoelectric point:

6.186

Motif(s):

Type	Positions	Sequence	Comment
	15 -> 367	QLIEHLRERASHLSYTAERPLRIMEVCGGHTHAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRVPGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQAKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDFHRPLVVAGFEPLDLLQGVVMLVQQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCVNGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPHQCPLFGNTCNPQTAFGALMVSSEGACAAWYQYR
	41 -> 41	C	UniProt: Does not affect interaction with HypC. Retains the 4Fe-4S cluster, but has less iron than the native complex. Can no longer coordinate CN(-1) and CO..
	69 -> 69	C	UniProt: Can no longer coordinate CN(-1) and CO. Reduces iron content..
	72 -> 72	C	UniProt: Can no longer coordinate CN(-1) and CO. Reduces iron content..
	357 -> 357	E	UniProt: Does not affect CN(-1) and CO coordination..

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
		2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0016887 - ATP hydrolysis activity GO:0046872 - metal ion binding GO:0051536 - iron-sulfur cluster binding GO:0051539 - 4 iron, 4 sulfur cluster binding GO:0005506 - iron ion binding GO:0070025 - carbon monoxide binding
	biological_process	GO:0006464 - cellular protein modification process GO:0051604 - protein maturation

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Jacobi A., et al., 1992
[2] Sankar P., et al., 1988
[3] Stoker K., et al., 1989
[4] Stripp ST., et al., 2014

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	FhlA, FNR, IHF
Repressed by:	NsrR

Reference(s)
[1] Jacobi A., Rossmann R., Bock A., 1992, The hyp operon gene products are required for the maturation of catalytically active hydrogenase isoenzymes in Escherichia coli., Arch Microbiol 158(6):444-51
[2] Sankar P., Shanmugam KT., 1988, Hydrogen metabolism in Escherichia coli: biochemical and genetic evidence for a hydF gene., J Bacteriol 170(12):5446-51
[3] Stoker K., Oltmann LF., Stouthamer AH., 1989, Randomly induced Escherichia coli K-12 Tn5 insertion mutants defective in hydrogenase activity., J Bacteriol 171(2):831-6
[4] Stripp ST., Lindenstrauss U., Granich C., Sawers RG., Soboh B., 2014, The influence of oxygen on [NiFe]-hydrogenase cofactor biosynthesis and how ligation of carbon monoxide precedes cyanation., PLoS One 9(9):e107488