RegulonDB RegulonDB 11.1: Gene Form
   

kdpA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

kdpB kdpA ybfA kdpF KdpE anti-anti-terminator anti-anti-terminator anti-terminator terminator terminator terminator TSS_830 TSS_830 ybfAp8 ybfAp8 TSS_828 (cluster) TSS_828 (cluster) ybfAp7 ybfAp7 kdpFp kdpFp

Gene      
Name: kdpA    Texpresso search in the literature
Synonym(s): ECK0686, EG10513, b0698, kac
Genome position(nucleotides): 727059 <-- 728732
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.5
External database links:  
ASAP:
 ABE-0002381
CGSC:
 588
ECHOBASE:
 EB0508
ECOLIHUB:
 kdpA
OU-MICROARRAY:
 b0698
STRING:
 511145.b0698
COLOMBOS: kdpA


Product      
Name: K+ transporting P-type ATPase subunit KdpA
Synonym(s): Kac, KdpA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 59.189
Isoelectric point: 7.468
Motif(s):
 
Type Positions Sequence Comment
5 -> 25 GFLLIATFLLVLMVLARPLGS UniProt: Helical.
11 -> 556 TFLLVLMVLARPLGSGLARLINDIPLPGTTGVERVLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLALNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSMSTLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGPVASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVMGDRRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGTDSSINMEGKESRFGVLVSSLFAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVFIAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGPHGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLVSKKSQAASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYL
63 -> 83 LCAILGLNMLGLAVLFFMLLG UniProt: Helical.
114 -> 114 N N → I: reduced affinity for K+; Km for K+ transport increased to 0.3 mM
116 -> 116 Q Q → R: reduced affinity for K+; Km for K+ transport increased to 6 mM

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.3 - The P-type ATPase (P-ATPase) Superfamily
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0031004 - potassium ion-transporting ATPase complex
GO:1903103 - potassium:proton antiporter complex
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005515 - protein binding
GO:0008556 - P-type potassium transmembrane transporter activity
GO:0030955 - potassium ion binding
biological_process GO:0006811 - ion transport
GO:0006813 - potassium ion transport
GO:0071805 - potassium ion transmembrane transport
GO:0098655 - cation transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Bertrand J., et al., 2004
[2] Dorus S., et al., 2001
[3] Sardesai AA., et al., 2001
[4] Schrader M., et al., 2000
External database links:  
ALPHAFOLD:
 P03959
ECOCYC:
 EG10513-MONOMER
ECOLIWIKI:
 b0698
INTERPRO:
 IPR004623
PANTHER:
 PTHR30607
PDB:
 7LC3
PDB:
 7LC6
PDB:
 7BH2
PDB:
 7BH1
PDB:
 7BGY
PDB:
 6HRB
PDB:
 5MRW
PDB:
 6HRA
PFAM:
 PF03814
PRIDE:
 P03959
PRODB:
 PRO_000023050
REFSEQ:
 NP_415226
SMR:
 P03959
UNIPROT:
 P03959


Operon      
Name: kdpFABC         
Operon arrangement:
Transcription unit        Promoter
kdpFABC


Transcriptional Regulation      
Display Regulation             
Activated by: KdpE


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybfAp7 729037 forward nd [COMP-AINF] [5]
  promoter TSS_828 (cluster) 729071 forward nd [RS-EPT-CBR] [6]
  promoter ybfAp8 729082 forward nd [COMP-AINF], [RS-EPT-CBR] [5], [6]
  promoter TSS_830 729088 forward nd [RS-EPT-CBR] [6]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Bertrand J., Altendorf K., Bramkamp M., 2004, Amino acid substitutions in putative selectivity filter regions III and IV in KdpA alter ion selectivity of the KdpFABC complex from Escherichia coli., J Bacteriol 186(16):5519-22

 [2] Dorus S., Mimura H., Epstein W., 2001, Substrate-binding clusters of the K+-transporting Kdp ATPase of Escherichia coli investigated by amber suppression scanning mutagenesis., J Biol Chem 276(13):9590-8

 [3] Sardesai AA., Gowrishankar J., 2001, Improvement in K+-limited growth rate associated with expression of the N-terminal fragment of one subunit (KdpA) of the multisubunit Kdp transporter in Escherichia coli., J Bacteriol 183(11):3515-20

 [4] Schrader M., Fendler K., Bamberg E., Gassel M., Epstein W., Altendorf K., Drose S., 2000, Replacement of glycine 232 by aspartic acid in the KdpA subunit broadens the ion specificity of the K(+)-translocating KdpFABC complex., Biophys J 79(2):802-13

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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