Gene
Name:	rsmA
Synonym(s):	ECK0052, EG10523, b0051, ksgA
Genome position(nucleotides):	51609 <-- 52430
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.04
External database links:

ASAP:	ABE-0000175
CGSC:	583
ECHOBASE:	EB0518
ECOLIHUB:	ksgA
OU-MICROARRAY:	b0051
STRING:	511145.b0051
COLOMBOS:	rsmA

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	apaHp4	51293	reverse	nd	[COMP-AINF]	[14]
promoter	TSS_84	51998	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_86	52194	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_87 (cluster)	52478	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_88	52548	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_89	52587	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_90	53904	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_91	54275	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_92	54363	reverse	nd	[RS-EPT-CBR]	[15]
promoter	TSS_93	54605	reverse	nd	[RS-EPT-CBR]	[15]

Evidence
[COMP-AINF] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Andresson OS., Davies JE., 1980, Genetic organization and restriction enzyme cleavage map of the ksgA-pdxA region of the Escherichia coli chromosome., Mol Gen Genet 179(1):211-6
[2] Andresson OS., Davies JE., 1980, Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA., Mol Gen Genet 179(1):201-9
[3] Chen J., Acton TB., Basu SK., Montelione GT., Inouye M., 2002, Enhancement of the solubility of proteins overexpressed in Escherichia coli by heat shock., J Mol Microbiol Biotechnol 4(6):519-24
[4] Helser TL., Davies JE., Dahlberg JE., 1971, Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli., Nat New Biol 233(35):12-4
[5] Helser TL., Davies JE., Dahlberg JE., 1972, Mechanism of kasugamycin resistance in Escherichia coli., Nat New Biol 235(53):6-9
[6] Kang TM., Yuan J., Nguyen A., Becket E., Yang H., Miller JH., 2012, The aminoglycoside antibiotic kanamycin damages DNA bases in Escherichia coli: caffeine potentiates the DNA-damaging effects of kanamycin while suppressing cell killing by ciprofloxacin in Escherichia coli and Bacillus anthracis., Antimicrob Agents Chemother 56(6):3216-23
[7] O'Farrell HC., Rife JP., 2012, Staphylococcus aureus and Escherichia coli have disparate dependences on KsgA for growth and ribosome biogenesis., BMC Microbiol 12:244
[8] Pulicherla N., Pogorzala LA., Xu Z., O Farrell HC., Musayev FN., Scarsdale JN., Sia EA., Culver GM., Rife JP., 2009, Structural and functional divergence within the Dim1/KsgA family of rRNA methyltransferases., J Mol Biol 391(5):884-93
[9] Roa BB., Connolly DM., Winkler ME., 1989, Overlap between pdxA and ksgA in the complex pdxA-ksgA-apaG-apaH operon of Escherichia coli K-12., J Bacteriol 171(9):4767-77
[10] Sparling PF., Ikeya Y., Elliot D., 1973, Two genetic loci for resistance to kasugamycin in Escherichia coli., J Bacteriol 113(2):704-10
[11] Stephan NC., Ries AB., Boehringer D., Ban N., 2021, Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA., Nucleic Acids Res 49(11):6389-6398
[12] van Buul CP., van Knippenberg PH., 1985, Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA., Gene 38(1-3):65-72
[13] van Gemen B., Koets HJ., Plooy CA., Bodlaender J., Van Knippenberg PH., 1987, Characterization of the ksgA gene of Escherichia coli determining kasugamycin sensitivity., Biochimie 69(8):841-8
[14] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[15] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Transcriptional Regulation
	Display Regulation
Activated by:	Fis