RegulonDB RegulonDB 11.1: Gene Form
   

manZ gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

manZ manY yobD terminator anti-terminator anti-anti-terminator TSS_2171 TSS_2171 TSS_2170 TSS_2170 TSS_2169 TSS_2169 TSS_2168 TSS_2168 TSS_2167 TSS_2167 TSS_2166 TSS_2166 TSS_2165 (cluster) TSS_2165 (cluster) TSS_2164 TSS_2164 TSS_2163 TSS_2163 TSS_2162 TSS_2162 TSS_2161 TSS_2161 TSS_2160 (cluster) TSS_2160 (cluster) TSS_2159 TSS_2159 TSS_2158 TSS_2158 TSS_2157 TSS_2157 TSS_2156 (cluster) TSS_2156 (cluster) TSS_2155 TSS_2155 TSS_2154 TSS_2154 TSS_2153 TSS_2153 TSS_2152 TSS_2152 TSS_2151 TSS_2151 TSS_2150 TSS_2150

Gene      
Name: manZ    Texpresso search in the literature
Synonym(s): ECK1817, EG10569, b1819, gptB, mpt, ptsM, ptsX
Genome position(nucleotides): 1903895 --> 1904746
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.7
External database links:  
ASAP:
 ABE-0006058
CGSC:
 346
ECHOBASE:
 EB0564
ECOLIHUB:
 manZ
OU-MICROARRAY:
 b1819
STRING:
 511145.b1819
COLOMBOS: manZ


Product      
Name: mannose-specific PTS enzyme IID component
Synonym(s): Enzyme IIDman, GptB, II-Mman, IIDMan, ManZ, Mpt, PtsM, PtsX
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 30.955
Isoelectric point: 9.648
Motif(s):
 
Type Positions Sequence Comment
1 -> 175 MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGID substrate binding
11 -> 281 KKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLL UniProt: PTS EIID.
12 -> 281 KLTQSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDGAINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLATRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSRITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGYACGLL
15 -> 52 QSDIRGVFLRSNLFQGSWNFERMQALGFCFSMVPAIRR
60 -> 92 ARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQ

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.4 - Group Translocators --> 4.4.A - Phosphotransferase Systems (PEP-dependent PTS)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:1902495 - transmembrane transporter complex
molecular_function GO:0022870 - protein-N(PI)-phosphohistidine-mannose phosphotransferase system transporter activity
biological_process GO:0098708 - glucose import across plasma membrane
GO:0008643 - carbohydrate transport
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system
GO:0015764 - N-acetylglucosamine transport
GO:0015761 - mannose transmembrane transport
GO:0032445 - fructose import
Note(s): Note(s): ...[more].
Reference(s): [1] Rhiel E., et al., 1994
External database links:  
ALPHAFOLD:
 P69805
ECOCYC:
 MANZ-MONOMER
ECOLIWIKI:
 b1819
INTERPRO:
 IPR004704
PDB:
 6K1H
PFAM:
 PF03613
PRIDE:
 P69805
PRODB:
 PRO_000023163
PROSITE:
 PS51108
REFSEQ:
 NP_416333
SMR:
 P69805
UNIPROT:
 P69805


Operon      
Name: manXYZ         
Operon arrangement:
Transcription unit        Promoter
manXYZ


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: NagC, Mlc, Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2150 1903166 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2151 1903202 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2152 1903244 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2153 1903321 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2154 1903409 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2155 1903587 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2156 (cluster) 1903744 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2157 1903789 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2158 1903802 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2159 1903806 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2160 (cluster) 1903812 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2161 1903815 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2162 1903818 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2163 1903821 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2164 1903823 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2165 (cluster) 1903860 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2166 1903863 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2167 1903867 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2168 1903873 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2169 1903946 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2170 1903975 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2171 1904505 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Rhiel E., Flukiger K., Wehrli C., Erni B., 1994, The mannose transporter of Escherichia coli K12: oligomeric structure, and function of two conserved cysteines., Biol Chem Hoppe Seyler 375(8):551-9

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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