RegulonDB RegulonDB 11.1: Gene Form
   

ptsI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ptsI crr ptsH TSS_2732 TSS_2732 TSS_2731 TSS_2731 TSS_2730 (cluster) TSS_2730 (cluster) TSS_2729 (cluster) TSS_2729 (cluster) TSS_2728 (cluster) TSS_2728 (cluster) TSS_2727 TSS_2727 TSS_2726 (cluster) TSS_2726 (cluster) TSS_2725 TSS_2725 TSS_2724 TSS_2724 TSS_2723 TSS_2723 TSS_2722 TSS_2722 TSS_2721 TSS_2721 TSS_2720 TSS_2720 TSS_2719 (cluster) TSS_2719 (cluster) crrpI crrpI crrp2 crrp2 TSS_2718 TSS_2718 TSS_2717 TSS_2717 TSS_2716 TSS_2716 TSS_2715 TSS_2715 TSS_2714 TSS_2714 TSS_2713 TSS_2713 TSS_2712 TSS_2712 TSS_2711 (cluster) TSS_2711 (cluster) TSS_2710 TSS_2710 TSS_2709 TSS_2709 TSS_2708 TSS_2708

Gene      
Name: ptsI    Texpresso search in the literature
Synonym(s): ECK2411, EG10789, b2416, ctr
Genome position(nucleotides): 2534066 --> 2535793
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.65
External database links:  
ASAP:
 ABE-0007967
CGSC:
 347
ECHOBASE:
 EB0782
ECOLIHUB:
 ptsI
OU-MICROARRAY:
 b2416
STRING:
 511145.b2416
COLOMBOS: ptsI


Product      
Name: PTS enzyme I
Synonym(s): Ctr, EI, PtsI
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 63.562
Isoelectric point: 4.495
Motif(s):
 
Type Positions Sequence Comment
1 -> 20 MISGILASPGIAFGKALLLK
4 -> 126 GILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFLSGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQASALEELDDEYLKER
21 -> 30 EDEIVIDRKK amino acid residues 21-30 and 44-148 constitute linkers between the HPr binding and His domains
31 -> 143 ISADQVDQEVERFLSGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADAAAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILG
122 -> 122 Y Y → F: loss of polar localization; does not affect sugar transport nor the ability to interact physically with TmaR

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  4 - transport --> 4.8.A - Accessory Factors Involved in Transport
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0016772 - transferase activity, transferring phosphorus-containing groups
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0008965 - phosphoenolpyruvate-protein phosphotransferase activity
GO:0042802 - identical protein binding
biological_process GO:0008643 - carbohydrate transport
GO:0009401 - phosphoenolpyruvate-dependent sugar phosphotransferase system
GO:0016310 - phosphorylation
External database links:  
ALPHAFOLD:
 P08839
DIP:
 DIP-10603N
ECOCYC:
 PTSI-MONOMER
ECOLIWIKI:
 b2416
INTERPRO:
 IPR036637
INTERPRO:
 IPR040442
INTERPRO:
 IPR036618
INTERPRO:
 IPR024692
INTERPRO:
 IPR023151
INTERPRO:
 IPR018274
INTERPRO:
 IPR015813
INTERPRO:
 IPR008731
INTERPRO:
 IPR008279
INTERPRO:
 IPR006318
INTERPRO:
 IPR000121
MINT:
 P08839
MODBASE:
 P08839
PDB:
 6V9K
PDB:
 6VU0
PDB:
 1ZYM
PDB:
 3EZB
PDB:
 3EZE
PDB:
 2EZA
PDB:
 2EZB
PDB:
 2EZC
PDB:
 2HWG
PDB:
 2KX9
PDB:
 2L5H
PDB:
 2MP0
PDB:
 2N5T
PDB:
 2XDF
PDB:
 3EZA
PDB:
 1EZD
PDB:
 1EZC
PDB:
 1EZB
PDB:
 1EZA
PFAM:
 PF02896
PFAM:
 PF00391
PFAM:
 PF05524
PRIDE:
 P08839
PRODB:
 PRO_000023633
PROSITE:
 PS00742
PROSITE:
 PS00370
REFSEQ:
 NP_416911
SMR:
 P08839
SWISSMODEL:
 P08839
UNIPROT:
 P08839


Operon      
Name: ptsHI-crr         
Operon arrangement:
Transcription unit        Promoter
ptsHI-crr
ptsHI-crr
ptsHI-crr
ptsHI-crr
ptsHI-crr
ptsHI-crr
crr
crr


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, Cra
Repressed by: CRP, NagC, Mlc, Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2708 2533930 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2709 2533978 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2710 2534042 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2711 (cluster) 2534053 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2712 2534171 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2713 2534767 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2714 2534771 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2715 2534776 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2716 2535322 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2717 2535452 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2718 2535474 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2719 (cluster) 2535671 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2720 2535696 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2721 2535734 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2722 2535767 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2723 2535778 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2724 2535782 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2725 2535785 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2726 (cluster) 2535792 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2727 2535802 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2728 (cluster) 2535812 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2729 (cluster) 2535819 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2730 (cluster) 2535831 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2731 2535839 forward nd [RS-EPT-CBR] [1]
  promoter TSS_2732 2536064 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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