RegulonDB

Gene
Name:	xylA
Synonym(s):	ECK3554, EG11074, b3565
Genome position(nucleotides):	3729443 <-- 3730765
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.87
External database links:

ASAP:	ABE-0011639
CGSC:	5
ECHOBASE:	EB1067
ECOLIHUB:	xylA
OU-MICROARRAY:	b3565
STRING:	511145.b3565
COLOMBOS:	xylA

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, XylR
Repressed by:	AraC

Reference(s)
[1] Ackerman RS., Cozzarelli NR., Epstein W., 1974, Accumulation of toxic concentrations of methylglyoxal by wild-type Escherichia coli K-12., J Bacteriol 119(2):357-62
[2] Alkim C., Cam Y., Trichez D., Auriol C., Spina L., Vax A., Bartolo F., Besse P., Francois JM., Walther T., 2015, Optimization of ethylene glycol production from (D)-xylose via a synthetic pathway implemented in Escherichia coli., Microb Cell Fact 14:127
[3] Batt CA., O'Neill E., Novak SR., Ko J., Sinskey A., 1986, Hyperexpression of Escherichia coli Xylose Isomerase., Biotechnol Prog 2(3):140-4
[4] Chen X., Wang W., Xu J., Yuan Z., Yuan T., Zhang Y., Liang C., He M., Guo Y., 2017, Production of d-psicose from d-glucose by co-expression of d-psicose 3-epimerase and xylose isomerase., Enzyme Microb Technol 105:18-23
[5] David JD., Wiesmeyer H., 1970, Control of xylose metabolism in Escherichia coli., Biochim Biophys Acta 201(3):497-9
[6] Dien BS., Nichols NN., Bothast RJ., 2002, Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L-lactic acid., J Ind Microbiol Biotechnol 29(5):221-7
[7] Dmytruk OV., Voronovsky AY., Abbas CA., Dmytruk KV., Ishchuk OP., Sibirny AA., 2008, Overexpression of bacterial xylose isomerase and yeast host xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha., FEMS Yeast Res 8(1):165-73
[8] Eiteman MA., Lee SA., Altman E., 2008, A co-fermentation strategy to consume sugar mixtures effectively., J Biol Eng 2:3
[9] Eiteman MA., Lee SA., Altman R., Altman E., 2009, A substrate-selective co-fermentation strategy with Escherichia coli produces lactate by simultaneously consuming xylose and glucose., Biotechnol Bioeng 102(3):822-7
[10] Hou YM., Zhang QJ., Wang SJ., 1990, Sequence analysis of D-xylose isomerase gene from Escherichia coli., Chin J Biotechnol 6(4):269-77
[11] Huang JJ., Ho NW., 1985, Cloning and expression of the Escherichia coli D-xylose isomerase gene in Bacillus subtilis., Biochem Biophys Res Commun 126(3):1154-60
[12] Kaup B., Bringer-Meyer S., Sahm H., 2005, D: -Mannitol formation from D: -glucose in a whole-cell biotransformation with recombinant Escherichia coli., Appl Microbiol Biotechnol 69(4):397-403
[13] Kawaguchi H., Vertes AA., Okino S., Inui M., Yukawa H., 2006, Engineering of a xylose metabolic pathway in Corynebacterium glutamicum., Appl Environ Microbiol 72(5):3418-28
[14] Kim SM., Choi BY., Ryu YS., Jung SH., Park JM., Kim GH., Lee SK., 2015, Simultaneous utilization of glucose and xylose via novel mechanisms in engineered Escherichia coli., Metab Eng 30:141-8
[15] Maleszka R., Wang PY., Schneider H., 1982, A Col E1 hybrid plasmid containing Escherichia coli genes complementing d-xylose negative mutants of Escherichia coli and Salmonella typhimurium., Can J Biochem 60(2):144-51
[16] Matsuoka Y., Shimizu K., 2013, Catabolite regulation analysis of Escherichia coli for acetate overflow mechanism and co-consumption of multiple sugars based on systems biology approach using computer simulation., J Biotechnol 168(2):155-73
[17] Meijnen JP., de Winde JH., Ruijssenaars HJ., 2008, Engineering Pseudomonas putida S12 for efficient utilization of D-xylose and L-arabinose., Appl Environ Microbiol 74(16):5031-7
[18] Rozanov AS., Zagrebel'nyi SN., Beklemishchev AB., 2009, [Cloning of Escherichia coli K12 xylose isomerase (glucose isomerase) and studying the enzymatic properties of its expression product]., Prikl Biokhim Mikrobiol 45(1):38-44
[19] Sapunova LI., Lobanok AG., Kazakevich IO., Shliakhotko EA., Evtushenkov AN., 2006, [Biosynthetic features and properties of xylose isomerases from Arthrobacter nicotianae, Escherichia coli, and Erwinia carotovota subsp. atroseptica]., Prikl Biokhim Mikrobiol 42(3):279-84
[20] Sarthy AV., McConaughy BL., Lobo Z., Sundstrom JA., Furlong CE., Hall BD., 1987, Expression of the Escherichia coli xylose isomerase gene in Saccharomyces cerevisiae., Appl Environ Microbiol 53(9):1996-2000
[21] Stevis PE., Ho NW., 1990, Inexpensive direct selection cloning system based on the xylA gene and MacConkey indicator plates., Biotechniques 9(6):703-4
[22] Stevis PE., Ho NW., 1987, Positive selection vectors based on xylose utilization suppression., Gene 55(1):67-74
[23] Tao H., Gonzalez R., Martinez A., Rodriguez M., Ingram LO., Preston JF., Shanmugam KT., 2001, Engineering a homo-ethanol pathway in Escherichia coli: increased glycolytic flux and levels of expression of glycolytic genes during xylose fermentation., J Bacteriol 183(10):2979-88
[24] Voronovsky AY., Ryabova OB., Verba OV., Ishchuk OP., Dmytruk KV., Sibirny AA., 2005, Expression of xylA genes encoding xylose isomerases from Escherichia coli and Streptomyces coelicolor in the methylotrophic yeast Hansenula polymorpha., FEMS Yeast Res 5(11):1055-62
[25] Wang X., Goh EB., Beller HR., 2018, Engineering E. coli for simultaneous glucose-xylose utilization during methyl ketone production., Microb Cell Fact 17(1):12
[26] Xia T., Eiteman MA., Altman E., 2012, Simultaneous utilization of glucose, xylose and arabinose in the presence of acetate by a consortium of Escherichia coli strains., Microb Cell Fact 11:77