RegulonDB RegulonDB 11.1: Gene Form
   

mtn gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dgt btuF mtn yadS CpxR dgtp2 dgtp2 dgtp1 dgtp1 mtnp2 mtnp2 mtnp mtnp TSS_302 TSS_302 TSS_301 TSS_301 yadSp5 yadSp5 yadSp7 yadSp7 yadSp6 yadSp6

Gene      
Name: mtn    Texpresso search in the literature
Synonym(s): ECK0158, EG11090, b0159, mtnN, pfs, yadA
Genome position(nucleotides): 178455 <-- 179153
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.36
External database links:  
ASAP:
 ABE-0000545
ECHOBASE:
 EB1082
ECOLIHUB:
 mtn
OU-MICROARRAY:
 b0159
STRING:
 511145.b0159
COLOMBOS: mtn


Product      
Name: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Synonym(s): Mtn, MtnN, Pfs, YadA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 24.354
Isoelectric point: 4.902
Motif(s):
 
Type Positions Sequence Comment
2 -> 226 KIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVALLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADVTAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAKIRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQSSLMVESLV
9 -> 9 M UniProt: 13-19% of wild-type catalytic efficiency..
12 -> 12 E UniProt: Loss of catalytic activity..
50 -> 50 I UniProt: 12-23% of wild-type catalytic efficiency..
76 -> 76 S UniProt: 13-23% of wild-type catalytic efficiency..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016787 - hydrolase activity
GO:0008782 - adenosylhomocysteine nucleosidase activity
GO:0008930 - methylthioadenosine nucleosidase activity
GO:0042802 - identical protein binding
GO:0042803 - protein homodimerization activity
biological_process GO:0110052 - toxic metabolite repair
GO:0008652 - cellular amino acid biosynthetic process
GO:0009086 - methionine biosynthetic process
GO:0009116 - nucleoside metabolic process
GO:0009164 - nucleoside catabolic process
GO:0019509 - L-methionine salvage from methylthioadenosine
GO:0019284 - L-methionine salvage from S-adenosylmethionine
GO:0033353 - S-adenosylmethionine cycle
GO:0046124 - purine deoxyribonucleoside catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Brown M., et al., 2021
[2] Kunjapur AM., et al., 2016
[3] Quan Y., et al., 2017
[4] Schauder S., et al., 2001
[5] Shen Y., et al., 2010
External database links:  
ALPHAFOLD:
 P0AF12
DIP:
 DIP-10270N
ECOCYC:
 EG11090-MONOMER
ECOLIWIKI:
 b0159
INTERPRO:
 IPR000845
INTERPRO:
 IPR010049
INTERPRO:
 IPR035994
MODBASE:
 P0AF12
PDB:
 4WKC
PDB:
 4YML
PDB:
 3O4V
PDB:
 1Z5P
PDB:
 1Z5O
PDB:
 1Z5N
PDB:
 1Y6R
PDB:
 1Y6Q
PDB:
 1NC3
PDB:
 1JYS
PDB:
 1NC1
PFAM:
 PF01048
PRIDE:
 P0AF12
PRODB:
 PRO_000023306
REFSEQ:
 NP_414701
SMR:
 P0AF12
SWISSMODEL:
 P0AF12
UNIPROT:
 P0AF12


Operon      
Name: mtn-btuF-yadS         
Operon arrangement:
Transcription unit        Promoter
mnt-btuF-yadS
mnt-btuF-yadS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yadSp6 177700 reverse nd [COMP-AINF] [6]
  promoter yadSp7 177757 reverse nd [COMP-AINF] [6]
  promoter yadSp5 177786 reverse nd [COMP-AINF] [6]
  promoter TSS_301 178744 reverse nd [RS-EPT-CBR] [7]
  promoter TSS_302 179181 reverse nd [RS-EPT-CBR] [7]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Brown M., Zoi I., Antoniou D., Namanja-Magliano HA., Schwartz SD., Schramm VL., 2021, Inverse heavy enzyme isotope effects in methylthioadenosine nucleosidases., Proc Natl Acad Sci U S A 118(40)

 [2] Kunjapur AM., Hyun JC., Prather KL., 2016, Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway., Microb Cell Fact 15:61

 [3] Quan Y., Meng F., Ma X., Song X., Liu X., Gao W., Dang Y., Meng Y., Cao M., Song C., 2017, Regulation of bacteria population behaviors by AI-2 "consumer cells" and "supplier cells"., BMC Microbiol 17(1):198

 [4] Schauder S., Shokat K., Surette MG., Bassler BL., 2001, The LuxS family of bacterial autoinducers: biosynthesis of a novel quorum-sensing signal molecule., Mol Microbiol 41(2):463-76

 [5] Shen Y., Liu J., Estiu G., Isin B., Ahn YY., Lee DS., Barabasi AL., Kapatral V., Wiest O., Oltvai ZN., 2010, Blueprint for antimicrobial hit discovery targeting metabolic networks., Proc Natl Acad Sci U S A 107(3):1082-7

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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