RegulonDB RegulonDB 11.1: Gene Form
   

rpmI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

infC rplT rpmI DksA-ppGpp DksA-ppGpp anti-anti-terminator anti-terminator terminator anti-terminator TSS_2022 TSS_2022 TSS_2021 TSS_2021 rpmIp rpmIp TSS_2020 (cluster) TSS_2020 (cluster) TSS_2019 TSS_2019 TSS_2018 TSS_2018 TSS_2017 TSS_2017 TSS_2016 TSS_2016 TSS_2015 TSS_2015 TSS_2014 (cluster) TSS_2014 (cluster) rplTp rplTp TSS_2013 TSS_2013

Gene      
Name: rpmI    Texpresso search in the literature
Synonym(s): ECK1715, EG11231, b1717
Genome position(nucleotides): 1799802 <-- 1799999
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.0
External database links:  
ASAP:
 ABE-0005732
CGSC:
 17602
ECHOBASE:
 EB1213
ECOLIHUB:
 rpmI
OU-MICROARRAY:
 b1717
STRING:
 511145.b1717
COLOMBOS: rpmI


Product      
Name: 50S ribosomal subunit protein L35
Synonym(s): 50S ribosomal subunit protein A, RpmI, X1 protein
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,ribosome
Molecular weight: 7.289
Isoelectric point: 12.325
Motif(s):
 
Type Positions Sequence Comment
1 -> 22 MPKIKTVRGAAKRFKKTGKGGF UniProt: Disordered; Sequence Annotation Type: region of interest.
2 -> 60 PKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHLRPKAMVSKGDLGLVIA
11 -> 11 A UniProt: In Ref. 1; V00291..
28 -> 28 N UniProt: In Ref. 5; AA sequence..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.2 - translation
  2 - information transfer --> 2.3 - protein related --> 2.3.8 - ribosomal proteins
  6 - cell structure --> 6.6 - ribosomes
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0005840 - ribosome
GO:0015934 - large ribosomal subunit
GO:0022625 - cytosolic large ribosomal subunit
molecular_function GO:0003735 - structural constituent of ribosome
biological_process GO:0006412 - translation
GO:0002181 - cytoplasmic translation
Note(s): Note(s): ...[more].
Reference(s): [1] Chiaruttini C., et al., 1996
[2] Lesage P., et al., 1992
[3] Wittmann-Liebold B., et al., 1995
External database links:  
ALPHAFOLD:
 P0A7Q1
ECOCYC:
 EG11231-MONOMER
ECOLIWIKI:
 b1717
INTERPRO:
 IPR037229
INTERPRO:
 IPR001706
INTERPRO:
 IPR018265
INTERPRO:
 IPR021137
PANTHER:
 PTHR33343
PDB:
 2J28
PDB:
 2RDO
PDB:
 3BBX
PDB:
 3J5L
PDB:
 3J7Z
PDB:
 3J8G
PDB:
 3J9Y
PDB:
 3J9Z
PDB:
 3JA1
PDB:
 3JBU
PDB:
 3JBV
PDB:
 3JCD
PDB:
 3JCE
PDB:
 3JCJ
PDB:
 3JCN
PDB:
 4CSU
PDB:
 4U1U
PDB:
 4U1V
PDB:
 4U20
PDB:
 4U24
PDB:
 4U25
PDB:
 4U26
PDB:
 4U27
PDB:
 4UY8
PDB:
 4V4H
PDB:
 4V4Q
PDB:
 4V50
PDB:
 4V52
PDB:
 4V53
PDB:
 4V54
PDB:
 4V55
PDB:
 4V56
PDB:
 4V57
PDB:
 4V5B
PDB:
 4V5H
PDB:
 4V5Y
PDB:
 4V64
PDB:
 4V65
PDB:
 4V66
PDB:
 4V69
PDB:
 4V6C
PDB:
 4V6D
PDB:
 4V6E
PDB:
 4V6K
PDB:
 4V6L
PDB:
 4V6M
PDB:
 4V6N
PDB:
 4V6O
PDB:
 4V6P
PDB:
 4V6Q
PDB:
 4V6R
PDB:
 4V6S
PDB:
 4V6T
PDB:
 4V6V
PDB:
 4V6Y
PDB:
 4V6Z
PDB:
 4V70
PDB:
 4V71
PDB:
 4V72
PDB:
 4V73
PDB:
 4V74
PDB:
 4V75
PDB:
 4V76
PDB:
 4V77
PDB:
 4V78
PDB:
 4V79
PDB:
 4V7A
PDB:
 4V7B
PDB:
 4V7C
PDB:
 4V7D
PDB:
 4V7I
PDB:
 4V7S
PDB:
 4V7T
PDB:
 4V7U
PDB:
 4V7V
PDB:
 4V85
PDB:
 4V89
PDB:
 4V9C
PDB:
 4V9D
PDB:
 4V9O
PDB:
 4V9P
PDB:
 4WF1
PDB:
 4WOI
PDB:
 4WWW
PDB:
 4YBB
PDB:
 5ADY
PDB:
 5AFI
PDB:
 5AKA
PDB:
 5GAD
PDB:
 5GAE
PDB:
 5GAF
PDB:
 5GAG
PDB:
 5GAH
PDB:
 5H5U
PDB:
 5IQR
PDB:
 5IT8
PDB:
 5J5B
PDB:
 5J7L
PDB:
 5J88
PDB:
 5J8A
PDB:
 5J91
PDB:
 5JC9
PDB:
 5JTE
PDB:
 5JU8
PDB:
 5KCR
PDB:
 5KCS
PDB:
 5KPS
PDB:
 5KPV
PDB:
 5KPW
PDB:
 5KPX
PDB:
 5L3P
PDB:
 5LZA
PDB:
 5LZB
PDB:
 5LZC
PDB:
 5LZD
PDB:
 5LZE
PDB:
 5LZF
PDB:
 5MDV
PDB:
 5MDW
PDB:
 5MDY
PDB:
 5MDZ
PDB:
 5MGP
PDB:
 5NCO
PDB:
 5NP6
PDB:
 5NWY
PDB:
 5O2R
PDB:
 5U4I
PDB:
 5U9F
PDB:
 5U9G
PDB:
 5UYK
PDB:
 5UYL
PDB:
 5UYM
PDB:
 5UYN
PDB:
 5UYP
PDB:
 5UYQ
PDB:
 5WDT
PDB:
 5WE4
PDB:
 5WE6
PDB:
 5WFK
PDB:
 6BU8
PDB:
 6BY1
PDB:
 6C4I
PDB:
 6ENF
PDB:
 6ENJ
PDB:
 6ENU
PDB:
 6GBZ
PDB:
 6GC0
PDB:
 6GC8
PDB:
 6GWT
PDB:
 6GXM
PDB:
 6GXN
PDB:
 6GXO
PDB:
 6GXP
PDB:
 6H4N
PDB:
 6H58
PDB:
 6HRM
PDB:
 6I0Y
PDB:
 6I7V
PDB:
 6O9J
PDB:
 6O9K
PDB:
 6OFX
PDB:
 6OG7
PDB:
 6ORE
PDB:
 6ORL
PDB:
 6OST
PDB:
 6OT3
PDB:
 6OUO
PDB:
 6Q97
PDB:
 6Q98
PDB:
 6Q9A
PDB:
 6QDW
PDB:
 6QUL
PDB:
 6S0K
PDB:
 6SZS
PDB:
 6TBV
PDB:
 6TC3
PDB:
 6VWL
PDB:
 6VWM
PDB:
 6VWN
PDB:
 6WD6
PDB:
 6WDB
PDB:
 6WDC
PDB:
 6WDD
PDB:
 6WDE
PDB:
 6WDF
PDB:
 6WDG
PDB:
 6WDH
PDB:
 6WDI
PDB:
 6WDJ
PDB:
 6WDK
PDB:
 6WDL
PDB:
 6WDM
PDB:
 6WNT
PDB:
 6WNV
PDB:
 6WNW
PDB:
 6XZ7
PDB:
 6XZA
PDB:
 6XZB
PDB:
 6Y69
PDB:
 6YS3
PDB:
 6YSR
PDB:
 6YSS
PDB:
 6YST
PDB:
 6YSU
PDB:
 6ZTJ
PDB:
 6ZTL
PDB:
 6ZTM
PDB:
 6ZTN
PDB:
 6ZTO
PDB:
 6ZTP
PDB:
 6ZU1
PDB:
 7BV8
PDB:
 7JSS
PDB:
 7JSW
PDB:
 7JSZ
PDB:
 7JT1
PDB:
 7JT2
PDB:
 7JT3
PFAM:
 PF01632
PRIDE:
 P0A7Q1
PRINTS:
 PR00064
PRODB:
 PRO_000023841
PROSITE:
 PS00936
REFSEQ:
 NP_416232
SMR:
 P0A7Q1
UNIPROT:
 P0A7Q1


Operon      
Name: thrS-infC-rpmI-rplT-pheMST-ihfA         
Operon arrangement:
Transcription unit        Promoter
ihfA
pheM
pheMST-ihfA
rplT
rplT-pheM
rpmI-rplT
infC-rpmI-rplT
infC
thrS-infC
thrS-infC-rpmI-rplT-pheMST-ihfA


Transcriptional Regulation      
Display Regulation             
Repressed by: NsrR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2013 1799621 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2014 (cluster) 1799992 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2015 1799997 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2016 1799999 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2017 1800003 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2018 1800005 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2019 1800009 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2020 (cluster) 1800331 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2021 1800430 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2022 1800596 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Chiaruttini C., Milet M., de Smit M., Springer M., 1996, Translational coupling in the Escherichia coli operon encoding translation initiation factor IF3 and ribosomal proteins L20 and L35., Biochimie 78(7):555-67

 [2] Lesage P., Chiaruttini C., Graffe M., Dondon J., Milet M., Springer M., 1992, Messenger RNA secondary structure and translational coupling in the Escherichia coli operon encoding translation initiation factor IF3 and the ribosomal proteins, L35 and L20., J Mol Biol 228(2):366-86

 [3] Wittmann-Liebold B., Uhlein M., Urlaub H., Muller EC., Otto A., Bischof O., 1995, Structural and functional implications in the eubacterial ribosome as revealed by protein-rRNA and antibiotic contact sites., Biochem Cell Biol 73(11-12):1187-97

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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