RegulonDB RegulonDB 11.1: Gene Form
   

nfrB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nfrA nfrB cusS ybcH cusR ybcHp4 ybcHp4

Gene      
Name: nfrB    Texpresso search in the literature
Synonym(s): ECK0561, EG11739, b0569
Genome position(nucleotides): 590941 <-- 593178
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.5
External database links:  
ASAP:
 ABE-0001942
CGSC:
 31157
ECHOBASE:
 EB1690
ECOLIHUB:
 nfrB
OU-MICROARRAY:
 b0569
STRING:
 511145.b0569
COLOMBOS: nfrB


Product      
Name: cyclic di-3',5'-guanylate-activated glycosyltransferase
Synonym(s): NfrB, bacteriophage N4 adsorption protein
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 85.319
Isoelectric point: 6.664
Motif(s):
 
Type Positions Sequence Comment
8 -> 28 FATWLYGLKVIAITLAVIMFI UniProt: Helical.
165 -> 382 FILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSELHGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKEKGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGIVFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLA
169 -> 169 D D → A: loss of phage N4 plaque forming ability
267 -> 267 D D → A: loss of phage N4 plaque forming ability
330 -> 330 W W → A: loss of phage N4 plaque forming ability

 
Classification:
Multifun Terms (GenProtEC)  
  6 - cell structure --> 6.1 - membrane
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0015627 - type II protein secretion system complex
molecular_function GO:0005515 - protein binding
GO:0016757 - glycosyltransferase activity
GO:0035438 - cyclic-di-GMP binding
biological_process GO:0045226 - extracellular polysaccharide biosynthetic process
GO:0015628 - protein secretion by the type II secretion system
Note(s): Note(s): ...[more].
Evidence: [EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Kiino DR., et al., 1989
[2] McPartland J., et al., 2009
External database links:  
ALPHAFOLD:
 P0AFA5
DIP:
 DIP-10333N
ECOCYC:
 EG11739-MONOMER
ECOLIWIKI:
 b0569
INTERPRO:
 IPR037257
INTERPRO:
 IPR029044
INTERPRO:
 IPR007831
INTERPRO:
 IPR001173
PFAM:
 PF05157
PFAM:
 PF13632
PRIDE:
 P0AFA5
PRODB:
 PRO_000023376
REFSEQ:
 NP_415101
SMR:
 P0AFA5
UNIPROT:
 P0AFA5


Operon      
Name: nfrBA         
Operon arrangement:
Transcription unit        Promoter
nfrBA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybcHp4 588070 reverse nd [COMP-AINF] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Kiino DR., Rothman-Denes LB., 1989, Genetic analysis of bacteriophage N4 adsorption., J Bacteriol 171(9):4595-602

 [2] McPartland J., Rothman-Denes LB., 2009, The tail sheath of bacteriophage N4 interacts with the Escherichia coli receptor., J Bacteriol 191(2):525-32

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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