RegulonDB RegulonDB 11.1: Gene Form
   

nfrA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nfrA nfrB cusS ybcH cusR ybcHp4 ybcHp4

Gene      
Name: nfrA    Texpresso search in the literature
Synonym(s): ECK0560, EG11740, b0568
Genome position(nucleotides): 587982 <-- 590954
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.06
External database links:  
ASAP:
 ABE-0001939
CGSC:
 31154
ECHOBASE:
 EB1691
ECOLIHUB:
 nfrA
OU-MICROARRAY:
 b0568
STRING:
 511145.b0568
COLOMBOS: nfrA


Product      
Name: exopolysaccharide secretion system outer membrane protein NfrA
Synonym(s): NfrA
Sequence: Get amino acid sequence Fasta Format
Cellular location: outer membrane
Molecular weight: 111.308
Isoelectric point: 7.0
Motif(s):
 
Type Positions Sequence Comment
1 -> 27 MKENNLNRVIGWSGLLLTSLLSTSALA
28 -> 990 DNIGTSAEELGLSDYRHFVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARLLLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNALRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLSAAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIENKPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDAAQKLLATLPANEMLEERYAVSVATRNKAEALRLARLLYQQEPANLTRLDQLTWQLMQNEQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQRQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQRQPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDRWLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQRHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEPAHKGLPDDPALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFIAVEQQLPLNGQNGASDTMLRASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAINQRNGERNNAFLTIGVHW UniProt: Bacteriophage adsorption protein A.
60 -> 123 NNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARLLLEDQLKRHPGDARLERSLAA
81 -> 114 IPLTLYLAEAYRHFGHDDRARLLLEDQLKRHPGD UniProt: TPR 1.
562 -> 704 AARDRWLQQAEKRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQRHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEPAHKGLPDDPALIRQLAYVNQRLDDMPATQHYAR

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport
  8 - extrachromosomal --> 8.1 - prophage genes and phage related functions
Gene Ontology Terms (GO)  
cellular_component GO:0009279 - cell outer membrane
GO:0016020 - membrane
biological_process GO:0032940 - secretion by cell
GO:0046813 - receptor-mediated virion attachment to host cell
Note(s): Note(s): ...[more].
Evidence: [EXP-IDA] Inferred from direct assay
[EXP-IMP] Inferred from mutant phenotype
Reference(s): [1] Junkermeier EH., et al., 2021
[2] Kiino DR., et al., 1989
External database links:  
ALPHAFOLD:
 P31600
DIP:
 DIP-10332N
ECOCYC:
 EG11740-MONOMER
ECOLIWIKI:
 b0568
INTERPRO:
 IPR019734
INTERPRO:
 IPR013026
INTERPRO:
 IPR011990
INTERPRO:
 IPR025137
MODBASE:
 P31600
PFAM:
 PF13283
PFAM:
 PF13429
PFAM:
 PF14559
PRIDE:
 P31600
PRODB:
 PRO_000023375
PROSITE:
 PS50005
PROSITE:
 PS50293
REFSEQ:
 NP_415100
SMART:
 SM00028
SMR:
 P31600
UNIPROT:
 P31600


Operon      
Name: nfrBA         
Operon arrangement:
Transcription unit        Promoter
nfrBA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybcHp4 588070 reverse nd [COMP-AINF] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Junkermeier EH., Hengge R., 2021, A Novel Locally c-di-GMP-Controlled Exopolysaccharide Synthase Required for Bacteriophage N4 Infection of Escherichia coli., mBio 12(6):e0324921

 [2] Kiino DR., Rothman-Denes LB., 1989, Genetic analysis of bacteriophage N4 adsorption., J Bacteriol 171(9):4595-602

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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