RegulonDB RegulonDB 11.1: Gene Form
   

purT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

purT eda yebG PurR LexA anti-anti-terminator anti-terminator terminator TSS_2193 TSS_2193 TSS_2192 TSS_2192 TSS_2191 TSS_2191 TSS_2190 TSS_2190 purTp purTp purTp3 purTp3 yebGp yebGp purTp5 purTp5

Gene      
Name: purT    Texpresso search in the literature
Synonym(s): ECK1850, EG11809, b1849
Genome position(nucleotides): 1930881 --> 1932059
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.77
External database links:  
ASAP:
 ABE-0006162
CGSC:
 32348
ECHOBASE:
 EB1757
ECOLIHUB:
 purT
OU-MICROARRAY:
 b1849
STRING:
 511145.b1849
COLOMBOS: purT


Product      
Name: phosphoribosylglycinamide formyltransferase 2
Synonym(s): 5'-phosphoribosylglycinamide transformylase 2, GAR transformylase 2, GAR transformylase T, GART2, PurT, glycinamide ribonucleotide transformylase 2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 42.434
Isoelectric point: 5.461
Motif(s):
 
Type Positions Sequence Comment
2 -> 392 TLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRYADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVVPCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSSSGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQNVTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG UniProt: Formate-dependent phosphoribosylglycinamide formyltransferase.
22 -> 23 EL UniProt: 5'-phosphoribosylglycinamide binding; Sequence Annotation Type: region of interest.
119 -> 308 RLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSSSGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEFALHVRAFL UniProt: ATP-grasp.
123 -> 294 EELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSSSGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLIS
160 -> 165 SSGKGQ UniProt: ATP.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.2 - nucleotides --> 1.5.2.1 - purine biosynthesis
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0016742 - hydroxymethyl-, formyl- and related transferase activity
GO:0004644 - phosphoribosylglycinamide formyltransferase activity
GO:0008776 - acetate kinase activity
GO:0000287 - magnesium ion binding
GO:0043815 - phosphoribosylglycinamide formyltransferase 2 activity
biological_process GO:0006164 - purine nucleotide biosynthetic process
GO:0009152 - purine ribonucleotide biosynthetic process
GO:0016310 - phosphorylation
GO:0006189 - 'de novo' IMP biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P33221
DIP:
 DIP-10618N
ECOCYC:
 GARTRANSFORMYL2-MONOMER
ECOLIWIKI:
 b1849
INTERPRO:
 IPR013815
INTERPRO:
 IPR016185
INTERPRO:
 IPR011761
INTERPRO:
 IPR011054
INTERPRO:
 IPR005862
INTERPRO:
 IPR003135
MODBASE:
 P33221
PDB:
 1KJI
PDB:
 1KJ8
PDB:
 1KJJ
PDB:
 1KJ9
PDB:
 1EZ1
PDB:
 1NFE
PDB:
 1EYZ
PDB:
 1KJQ
PFAM:
 PF02222
PRIDE:
 P33221
PRODB:
 PRO_000023645
PROSITE:
 PS50975
REFSEQ:
 NP_416363
SMR:
 P33221
UNIPROT:
 P33221


Operon      
Name: purT         
Operon arrangement:
Transcription unit        Promoter
purT


Transcriptional Regulation      
Display Regulation             
Repressed by: PurR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter purTp5 1930719 forward nd [COMP-AINF] [1]
  promoter purTp3 1930855 forward nd [COMP-AINF] [1]
  promoter TSS_2190 1931849 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2191 1932530 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2192 1932726 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2193 1932744 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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