Gene
Name:	amiA
Synonym(s):	ECK2430, EG11823, b2435, yfeE
Genome position(nucleotides):	2552352 --> 2553221 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	49.77
External database links:

ASAP:	ABE-0008032
CGSC:	18541
ECHOBASE:	EB1770
OU-MICROARRAY:	b2435
PortEco:	amiA
STRING:	511145.b2435
COLOMBOS:	amiA

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	yfeZp3	2551921		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 5.099. P-value: 7.560e-5 Read more >	[ICWHO]	[6]
	promoter	TSS_2747 (cluster)	2552242		forward	For this promoter, there is a cluster of 4 transcription start sites,in the interval 2552242 to 2552245, where the highest frequency is at position 2550264. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[7]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Heidrich C., Ursinus A., Berger J., Schwarz H., Holtje JV., 2002, Effects of multiple deletions of murein hydrolases on viability, septum cleavage, and sensitivity to large toxic molecules in Escherichia coli., J Bacteriol 184(22):6093-9
[2] Parquet C., Flouret B., Leduc M., Hirota Y., van Heijenoort J., 1983, N-acetylmuramoyl-L-alanine amidase of Escherichia coli K12. Possible physiological functions., Eur J Biochem 133(2):371-7
[3] Peters NT., Dinh T., Bernhardt TG., 2011, A fail-safe mechanism in the septal ring assembly pathway generated by the sequential recruitment of cell separation amidases and their activators., J Bacteriol 193(18):4973-83
[4] Uehara T., Park JT., 2008, Growth of Escherichia coli: significance of peptidoglycan degradation during elongation and septation., J Bacteriol 190(11):3914-22
[5] van Heijenoort J., Parquet C., Flouret B., van Heijenoort Y., 1975, Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme., Eur J Biochem 58(2):611-9
[6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Transcriptional Regulation
	Display Regulation
Repressed by:	IHF