RegulonDB

Gene
Name:	hscB
Synonym(s):	ECK2524, EG12131, b2527, yfhE
Genome position(nucleotides):	2658952 <-- 2659467 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.33
External database links:

ASAP:	ABE-0008317
ECHOBASE:	EB2052
ECOLIHUB:	hscB
OU-MICROARRAY:	b2527
STRING:	511145.b2527
COLOMBOS:	hscB

Product

Name:

[Fe-S] cluster biosynthesis co-chaperone HscB

Synonym(s):

Hsc20, Hsc20 co-chaperone that acts with Hsc66 in IscU iron-sulfur cluster assembly, HscB, YfhE, co-chaperone for [Fe-S] cluster biosynthesis

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

20.138

Isoelectric point:

4.825

Motif(s):

Type	Positions	Sequence
	88 -> 161	DTAFLMEQLELREELDEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSSA
	2 -> 74	DYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQLAAVQQSATINQAWQTLRHPLMRAEYLLSL

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.19 - incorporation of metal ions
		2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:1990230 - iron-sulfur cluster transfer complex
	molecular_function	GO:0005515 - protein binding GO:0051087 - chaperone binding GO:0001671 - ATPase activator activity
	biological_process	GO:0006457 - protein folding GO:0051259 - protein complex oligomerization GO:0032781 - positive regulation of ATPase activity GO:0097428 - protein maturation by iron-sulfur cluster transfer GO:0044571 - [2Fe-2S] cluster assembly

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Fuzery AK., et al., 2008
[2] Hoff KG., et al., 2003
[3] Hoff KG., et al., 2000
[4] Hoff KG., et al., 2002
[5] Takahashi Y., et al., 1999
[6] Tokumoto U., et al., 2004
[7] Tokumoto U., et al., 2001

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

hscBA-fdx-iscX

Operon arrangement:

Transcription unit	Promoter
hscBA-fdx-iscX	hscBp

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2825	2658287		reverse	nd	[RS-EPT-CBR]	[8]
	promoter	TSS_2826	2658293		reverse	nd	[RS-EPT-CBR]	[8]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Fuzery AK., Tonelli M., Ta DT., Cornilescu G., Vickery LE., Markley JL., 2008, Solution structure of the iron-sulfur cluster cochaperone HscB and its binding surface for the iron-sulfur assembly scaffold protein IscU., Biochemistry 47(36):9394-404
[2] Hoff KG., Cupp-Vickery JR., Vickery LE., 2003, Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system., J Biol Chem 278(39):37582-9
[3] Hoff KG., Silberg JJ., Vickery LE., 2000, Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli., Proc Natl Acad Sci U S A 97(14):7790-5
[4] Hoff KG., Ta DT., Tapley TL., Silberg JJ., Vickery LE., 2002, Hsc66 substrate specificity is directed toward a discrete region of the iron-sulfur cluster template protein IscU., J Biol Chem 277(30):27353-9
[5] Takahashi Y., Nakamura M., 1999, Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli., J Biochem 126(5):917-26
[6] Tokumoto U., Kitamura S., Fukuyama K., Takahashi Y., 2004, Interchangeability and distinct properties of bacterial Fe-S cluster assembly systems: functional replacement of the isc and suf operons in Escherichia coli with the nifSU-like operon from Helicobacter pylori., J Biochem (Tokyo) 136(2):199-209
[7] Tokumoto U., Takahashi Y., 2001, Genetic analysis of the isc operon in Escherichia coli involved in the biogenesis of cellular iron-sulfur proteins., J Biochem (Tokyo) 130(1):63-71
[8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.