RegulonDB RegulonDB 11.1: Gene Form
   

cobS gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cobT cobS cobU yeeH anti-anti-terminator anti-terminator terminator TSS_2305 TSS_2305 cobUp2 cobUp2 cobUp1 cobUp1 TSS_2304 TSS_2304 cobTp8 cobTp8

Gene      
Name: cobS    Texpresso search in the literature
Synonym(s): ECK1987, EG12150, b1992
Genome position(nucleotides): 2064479 <-- 2065222
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.49
External database links:  
ASAP:
 ABE-0006613
ECHOBASE:
 EB2070
ECOLIHUB:
 cobS
OU-MICROARRAY:
 b1992
STRING:
 511145.b1992
COLOMBOS: cobS


Product      
Name: cobalamin 5'-phosphate synthase
Synonym(s): CobS
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 26.386
Isoelectric point: 8.99
Motif(s):
 
Type Positions Sequence Comment
9 -> 244 LSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLLGAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELVFLL
34 -> 54 IITFPLIGLLLGAISGLVFMV UniProt: Helical.
48 -> 49 SG UniProt: In Ref. 1 and 5..
59 -> 79 CGAPLAALFSVLVLVLMTGGF UniProt: Helical.
113 -> 133 GGLALIFVVLAKILVLSELAL UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.13 - cobalamin
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0016740 - transferase activity
GO:0051073 - adenosylcobinamide-GDP ribazoletransferase activity
GO:0008818 - cobalamin 5'-phosphate synthase activity
biological_process GO:0009236 - cobalamin biosynthetic process
Note(s): Note(s): ...[more].
Evidence: [COMP-HINF-FN-FROM-SEQ] Human inference of function from sequence
[EXP-IGI-FUNC-COMPLEMENTATION] Inferred by functional complementation
Reference(s): [1] Lawrence JG., et al., 1995
External database links:  
ALPHAFOLD:
 P36561
ECOCYC:
 COBS-MONOMER
ECOLIWIKI:
 b1992
INTERPRO:
 IPR003805
PANTHER:
 PTHR34148
PFAM:
 PF02654
PRIDE:
 P36561
PRODB:
 PRO_000022313
REFSEQ:
 NP_416496
UNIPROT:
 P36561


Operon      
Name: cobUST         
Operon arrangement:
Transcription unit        Promoter
cobUST
cobUST


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter cobTp8 2064601 reverse nd [COMP-AINF] [2]
  promoter TSS_2304 2065847 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_2305 2065996 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Lawrence JG., Roth JR., 1995, The cobalamin (coenzyme B12) biosynthetic genes of Escherichia coli., J Bacteriol 177(22):6371-80

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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