RegulonDB RegulonDB 11.1: Gene Form
   

cobT gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cobT ldtA cobS cobU terminator cobTp8 cobTp8 erfKp4 erfKp4 TSS_2303 TSS_2303 TSS_2302 (cluster) TSS_2302 (cluster) TSS_2301 TSS_2301

Gene      
Name: cobT    Texpresso search in the literature
Synonym(s): ECK1986, EG12151, b1991
Genome position(nucleotides): 2063388 <-- 2064467
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.3
External database links:  
ASAP:
 ABE-0006611
ECHOBASE:
 EB2071
ECOLIHUB:
 cobT
OU-MICROARRAY:
 b1991
STRING:
 511145.b1991
COLOMBOS: cobT


Product      
Name: nicotinate-nucleotide—dimethylbenzimidazole phosphoribosyltransferase
Synonym(s): CobT
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 36.987
Isoelectric point: 4.991
Motif(s):
 
Type Positions Sequence Comment
11 -> 345 IPAIDSTAMSRAQRHIDGLLKPVGSLGKLEVLAIQLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVLAEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYTQELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVVRRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAACQMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACAIYNNMGELAAS
271 -> 271 A UniProt: In Ref. 1; AAA78908..
301 -> 301 L UniProt: In Ref. 1; AAA78908..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.13 - cobalamin
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016757 - glycosyltransferase activity
GO:0016740 - transferase activity
GO:0008939 - nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity
biological_process GO:0009236 - cobalamin biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P36562
ECOCYC:
 DMBPPRIBOSYLTRANS-MONOMER
ECOLIWIKI:
 b1991
INTERPRO:
 IPR017846
INTERPRO:
 IPR023195
INTERPRO:
 IPR003200
INTERPRO:
 IPR036087
MODBASE:
 P36562
PFAM:
 PF02277
PRIDE:
 P36562
PRODB:
 PRO_000022314
REFSEQ:
 NP_416495
SMR:
 P36562
SWISSMODEL:
 P36562
UNIPROT:
 P36562


Operon      
Name: cobUST         
Operon arrangement:
Transcription unit        Promoter
cobUST
cobUST


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2301 2062756 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2302 (cluster) 2062780 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2303 2063353 reverse nd [RS-EPT-CBR] [1]
  promoter erfKp4 2063354 reverse nd [COMP-AINF] [2]
  promoter cobTp8 2064601 reverse nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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