RegulonDB RegulonDB 11.1: Gene Form
   

rpnE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

glpC rpnE yfaU ypaA glpB terminator anti-terminator anti-anti-terminator yfaDp5 yfaDp5

Gene      
Name: rpnE    Texpresso search in the literature
Synonym(s): ECK2236, EG12323, b2244, yfaD
Genome position(nucleotides): 2356904 --> 2357803
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 48.33
External database links:  
ASAP:
 ABE-0007429
ECHOBASE:
 EB2228
ECOLIHUB:
 yfaD
OU-MICROARRAY:
 b2244
STRING:
 511145.b2244
COLOMBOS: rpnE


Product      
Name: inactive recombination-promoting nuclease-like protein RpnE
Synonym(s): RpnE, YfaD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 34.923
Isoelectric point: 5.003
Motif(s):
 
Type Positions Sequence Comment
8 -> 209 SPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQTLRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAAMQRHQDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFLLVDITIVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQL
86 -> 86 S UniProt: In Ref. 4; AAA83867..

 
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:1990238 - double-stranded DNA endodeoxyribonuclease activity
biological_process GO:0006310 - DNA recombination
Note(s): Note(s): ...[more].
Reference(s): [1] Meydan S., et al., 2019
External database links:  
ALPHAFOLD:
 P37014
ECOCYC:
 EG12323-MONOMER
ECOLIWIKI:
 b2244
INTERPRO:
 IPR006842
INTERPRO:
 IPR010106
PFAM:
 PF04754
PRIDE:
 P37014
REFSEQ:
 NP_416747
SMR:
 P37014
UNIPROT:
 P37014


Operon      
Name: rpnE-ypaA         
Operon arrangement:
Transcription unit        Promoter
rpnE-ypaA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yfaDp5 2356690 forward nd [COMP-AINF] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Meydan S., Marks J., Klepacki D., Sharma V., Baranov PV., Firth AE., Margus T., Kefi A., Vazquez-Laslop N., Mankin AS., 2019, Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome., Mol Cell 74(3):481-493.e6

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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