RegulonDB RegulonDB 11.1: Gene Form
   

cusA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cusA pheP cusB terminator anti-terminator anti-anti-terminator phePp phePp TSS_719 TSS_719 TSS_718 TSS_718 TSS_717 TSS_717 TSS_716 TSS_716 TSS_715 TSS_715

Gene      
Name: cusA    Texpresso search in the literature
Synonym(s): ECK0567, EG12367, b0575, ybdE
Genome position(nucleotides): 598714 --> 601857
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.69
External database links:  
ASAP:
 ABE-0001971
ECHOBASE:
 EB2270
ECOLIHUB:
 cusA
OU-MICROARRAY:
 b0575
STRING:
 511145.b0575
COLOMBOS: cusA


Product      
Name: copper/silver export system RND permease
Synonym(s): CusA, YbdE
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 114.707
Isoelectric point: 6.427
Motif(s):
 
Type Positions Sequence Comment
6 -> 1037 IRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIAELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITLADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKL
14 -> 34 FLVLMGALFLSIWGTWTIINT UniProt: Helical.
338 -> 358 LSGKLLEEFIVVAVVCALFLW UniProt: Helical.
363 -> 383 ALVAIISLPLGLCIAFIVMHF UniProt: Helical.
391 -> 411 MSLGGIAIAVGAMVDAAIVMI UniProt: Helical.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005507 - copper ion binding
GO:0005515 - protein binding
GO:0008324 - cation transmembrane transporter activity
GO:0005375 - copper ion transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0042910 - xenobiotic transmembrane transporter activity
GO:0015080 - silver ion transmembrane transporter activity
biological_process GO:0006811 - ion transport
GO:0006812 - cation transport
GO:0046688 - response to copper ion
GO:0009636 - response to toxic substance
GO:0006825 - copper ion transport
GO:0006878 - cellular copper ion homeostasis
GO:0055085 - transmembrane transport
GO:0010272 - response to silver ion
GO:0010273 - detoxification of copper ion
GO:0015673 - silver ion transport
GO:0015679 - plasma membrane copper ion transport
GO:0060003 - copper ion export
GO:0042908 - xenobiotic transport
GO:0035434 - copper ion transmembrane transport
GO:1902601 - silver ion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Silver S. 2003
[2] Yamada S., et al., 2006
[3] Yun BY., et al., 2010
External database links:  
ALPHAFOLD:
 P38054
DIP:
 DIP-9345N
ECOCYC:
 YBDE-MONOMER
ECOLIWIKI:
 b0575
INTERPRO:
 IPR027463
INTERPRO:
 IPR004763
INTERPRO:
 IPR001036
MODBASE:
 P38054
PANTHER:
 PTHR32063
PDB:
 3T53
PDB:
 4DNT
PDB:
 4DOP
PDB:
 3T51
PDB:
 3T56
PDB:
 4DNR
PDB:
 3K07
PDB:
 3K0I
PDB:
 3KSO
PDB:
 3KSS
PDB:
 3NE5
PFAM:
 PF00873
PRIDE:
 P38054
PRINTS:
 PR00702
PRODB:
 PRO_000022349
REFSEQ:
 NP_415107
SMR:
 P38054
UNIPROT:
 P38054


Operon      
Name: cusCFBA         
Operon arrangement:
Transcription unit        Promoter
cusCFBA


Transcriptional Regulation      
Display Regulation             
Activated by: HprR, PhoB, CusR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_715 597483 forward nd [RS-EPT-CBR] [4]
  promoter TSS_716 597539 forward nd [RS-EPT-CBR] [4]
  promoter TSS_717 597541 forward nd [RS-EPT-CBR] [4]
  promoter TSS_718 599136 forward nd [RS-EPT-CBR] [4]
  promoter TSS_719 600284 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Silver S., 2003, Bacterial silver resistance: molecular biology and uses and misuses of silver compounds., FEMS Microbiol Rev 27(2-3):341-53

 [2] Yamada S., Awano N., Inubushi K., Maeda E., Nakamori S., Nishino K., Yamaguchi A., Takagi H., 2006, Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli., Appl Environ Microbiol 72(7):4735-42

 [3] Yun BY., Xu Y., Piao S., Kim N., Yoon JH., Cho HS., Lee K., Ha NC., 2010, Periplasmic domain of CusA in an Escherichia coli Cu+/Ag+ transporter has metal binding sites., J Microbiol 48(6):829-35

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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