RegulonDB

RegulonDB RegulonDB 11.1: Gene Form

mdtM gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	mdtM
Synonym(s):	ECK4328, EG12576, b4337, yjiO
Genome position(nucleotides):	4567287 <-- 4568519
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.77
External database links:

ASAP:	ABE-0014221
ECHOBASE:	EB2464
ECOLIHUB:	mdtM
OU-MICROARRAY:	b4337
STRING:	511145.b4337
COLOMBOS:	mdtM

Product

Name:

multidrug efflux pump / bile salt:H⁺ antiporter / Na⁺:H⁺ antiporter / K⁺:H⁺ antiporter

Synonym(s):

MdtM, MdtM drug MFS transporter, YjiO, multidrug efflux pump MdtM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

44.688

Isoelectric point:

10.613

Motif(s):

Type	Positions	Sequence	Comment
	12 -> 32	LFFPMALILYDFAAYLSTDLI	UniProt: Helical.
	22 -> 22	D	D → A: binds TPP⁺ and chloramphenicol with wild-type affinity; loss of resistance to TPP⁺ and significant decrease in resistance to chloramphenical when overproduced in a ΔmdtM background
	23 -> 353	FAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLSLLIVGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFTLFSNKLPKGTVSASLNMVI
	26 -> 26	Y	Y → A: enhanced affinity for chloramphenicol and TPP⁺; increased resistance to chloramphenicol and TPP⁺ when overproduced in a ΔmdtM background
	49 -> 69	LAPAAVSLYLAGGMALQWLLG	UniProt: Helical.

Type	Positions	Sequence	Comment
	12 -> 32	LFFPMALILYDFAAYLSTDLI	UniProt: Helical.
	22 -> 22	D	D → A: binds TPP⁺ and chloramphenicol with wild-type affinity; loss of resistance to TPP⁺ and significant decrease in resistance to chloramphenical when overproduced in a ΔmdtM background
	23 -> 353	FAAYLSTDLIQPGIINVVRDFNADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMTQFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAALMHFMHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFLFGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVKDPTEPRFIWRAVPIQLVGLSLLIVGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFTLFSNKLPKGTVSASLNMVI
	26 -> 26	Y	Y → A: enhanced affinity for chloramphenicol and TPP⁺; increased resistance to chloramphenicol and TPP⁺ when overproduced in a ΔmdtM background
	49 -> 69	LAPAAVSLYLAGGMALQWLLG	UniProt: Helical.
	79 -> 99	PVLITGALIFTLACAATMFTT	UniProt: Helical.
	104 -> 124	FLIARAIQGTSICFIATVGYV	UniProt: Helical.
	116 -> 116	C	C → V: binds chloramphenicol with nM affinity; increased resistance to chloramphenicol when expressed in a ΔmdtM background
	119 -> 119	A	A → C: decreased affinity for TPP⁺; loss of resistance to TPP⁺ when overproduced in a ΔmdtM background; loss of TPP⁺ transport in inverted vesicles
	128 -> 128	E	UniProt: In strain: B..
	141 -> 161	IITSIVLIAPIIGPLSGAALM	UniProt: Helical.
	168 -> 188	VLFAIIAVMGFISFVGLLLAM	UniProt: Helical.
	217 -> 237	FLFGAATISLSYIPMMSWVAV	UniProt: Helical.
	252 -> 272	QFAWTQVPVFGAVIVANAIVA	UniProt: Helical.
	253 -> 253	F	F → A: binds TPP⁺ with nM affinity; increased resistance to TPP⁺ when overproduced in a ΔmdtM background; enhanced TPP⁺ transport in inverted vesicles
	257 -> 257	Q	Q → E: binds TPP⁺ with nM affinity; increased resistance to TPP⁺ when overproduced in a ΔmdtM background; enhanced TPP⁺ transport in inverted vesicles
	261 -> 261	F	F → A: binds TPP⁺ with wild-type affinity; loss of TPP⁺ resistance when overproduced in a ΔmdtM background; loss of TPP⁺ transport in inverted vesicles
	283 -> 303	FIWRAVPIQLVGLSLLIVGNL	UniProt: Helical.
	308 -> 328	VWLWSVLGTSLYAFGIGLIFP	UniProt: Helical.
	342 -> 342	K	K → E: binds TPP⁺ with enhanced affinity; loss of resistance to TPP⁺ when overproduced in a ΔmdtM background; retains residual TPP⁺ transport in inverted vesicles
	349 -> 369	LNMVILMVMSVSVEIGRWLWF	UniProt: Helical.
	374 -> 394	LPFHLLAVVAGVIVVFTLAGL	UniProt: Helical.

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015125 - bile acid transmembrane transporter activity GO:0022857 - transmembrane transporter activity GO:0015299 - solute:proton antiporter activity GO:0015385 - sodium:proton antiporter activity GO:0015386 - potassium:proton antiporter activity
	biological_process	GO:0097623 - potassium ion export across plasma membrane GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane GO:0036376 - sodium ion export across plasma membrane GO:0046677 - response to antibiotic GO:0030641 - regulation of cellular pH GO:0055085 - transmembrane transport GO:0015721 - bile acid and bile salt transport GO:0098656 - anion transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] Alegre KO., et al., 2015
[2] Alon Cudkowicz N., et al., 2019
[3] Edgar R., et al., 1997
[4] Jindal S., et al., 2019
[5] Schuldiner S. 2018
[6] Shuster Y., et al., 2016
[7] Tal N., et al., 2009

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

mdtM-yjiN

Operon arrangement:

Transcription unit	Promoter
mdtM-yjiN

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Alegre KO., Law CJ., 2015, Purification of a Multidrug Resistance Transporter for Crystallization Studies., Antibiotics (Basel) 4(1):113-35
[2] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828
[3] Edgar R., Bibi E., 1997, MdfA, an Escherichia coli multidrug resistance protein with an extraordinarily broad spectrum of drug recognition., J Bacteriol 179(7):2274-80
[4] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195
[5] Schuldiner S., 2018, The Escherichia coli effluxome., Res Microbiol 169(7-8):357-362
[6] Shuster Y., Steiner-Mordoch S., Alon Cudkowicz N., Schuldiner S., 2016, A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics., PLoS One 11(4):e0152917
[7] Tal N., Schuldiner S., 2009, A coordinated network of transporters with overlapping specificities provides a robust survival strategy., Proc Natl Acad Sci U S A 106(22):9051-6

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