RegulonDB RegulonDB 11.1: Gene Form
   

dgoA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dgoT dgoD dgoK dgoR dgoA cbrA terminator

Gene      
Name: dgoA    Texpresso search in the literature
Synonym(s): ECK3685, EG20049, b3692, b4477, yidU
Genome position(nucleotides): 3872995 <-- 3873612
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 59.39
External database links:  
ASAP:
 ABE-0174107
CGSC:
 36891
ECHOBASE:
 EB1667
ECOLIHUB:
 dgoA
OU-MICROARRAY:
 b3692
STRING:
 511145.b4477
COLOMBOS: dgoA


Product      
Name: 2-dehydro-3-deoxy-6-phosphogalactonate aldolase
Synonym(s): DgoA, YidU
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 21.391
Isoelectric point: 4.749
Motif(s):
 
Type Positions Sequence Comment
5 -> 183 TKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQWEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGMTVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPENLAQWIDAGCAGAGLGSDLYRAG
37 -> 37 E UniProt: 50-fold decrease in catalytic efficiency and 6-fold decrease of binding affinity..
154 -> 154 V UniProt: Little stereoselectivity, accepting KDPG and KDPGal as substrate with roughly equal efficacy. Reduced the preference for KDPGal. It diminishes the activity against KDPGal and increases activity against KDPG..
176 -> 177 GS UniProt: Substrate binding; Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0008674 - 2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
biological_process GO:0034194 - D-galactonate catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Ran N., et al., 2007
External database links:  
ALPHAFOLD:
 Q6BF16
ECOCYC:
 DEHYDDEOXPHOSGALACT-ALDOL-MONOMER
ECOLIWIKI:
 b4477
INTERPRO:
 IPR000887
INTERPRO:
 IPR013785
MODBASE:
 Q6BF16
PANTHER:
 PTHR30246
PDB:
 2V82
PDB:
 2V81
PDB:
 4QCC
PFAM:
 PF01081
PRIDE:
 Q6BF16
PRODB:
 PRO_000022433
REFSEQ:
 YP_026238
SMR:
 Q6BF16
UNIPROT:
 Q6BF16


Operon      
Name: dgoRKADT         
Operon arrangement:
Transcription unit        Promoter
dgoRKADT


Transcriptional Regulation      
Display Regulation             
Activated by: CRP
Repressed by: DgoR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Ran N., Frost JW., 2007, Directed evolution of 2-keto-3-deoxy-6-phosphogalactonate aldolase to replace 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase., J Am Chem Soc 129(19):6130-9

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