RegulonDB RegulonDB 10.10: Gene Form
   

ispU gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dxr cdsA uppS rsePp rsePp cdsAp2 cdsAp2 cdsAp3 cdsAp3 TSS_337 TSS_337 TSS_336 TSS_336 TSS_335 (cluster) TSS_335 (cluster) TSS_334 TSS_334 ispUp2 ispUp2 ispUp1 ispUp1 TSS_333 TSS_333

Gene      
Name: ispU    Texpresso search in the literature
Synonym(s): ECK0173, G6092, b0174, rth, uppS, yaeS
Genome position(nucleotides): 194903 --> 195664 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.0
External database links:  
ASAP:
 ABE-0000595
ECHOBASE:
 EB3113
ECOLIHUB:
 ispU
MIM:
 613861
OU-MICROARRAY:
 b0174
STRING:
 511145.b0174
COLOMBOS: ispU


Product      
Name: ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]
Synonym(s): IspU, Rth, UppS, YaeS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 28.444
Isoelectric point: 6.971
Motif(s):
 
Type Positions Sequence
31 -> 31 W
71 -> 73 SSE
73 -> 73 E
74 -> 74 N
77 -> 77 R

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.7 - murein (peptidoglycan)
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0004659 - prenyltransferase activity
GO:0008834 - di-trans,poly-cis-decaprenylcistransferase activity
GO:0002094 - polyprenyltransferase activity
GO:0000287 - magnesium ion binding
GO:0030145 - manganese ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0051301 - cell division
GO:0007049 - cell cycle
GO:0009252 - peptidoglycan biosynthetic process
GO:0043164 - Gram-negative-bacterium-type cell wall biogenesis
GO:0016094 - polyprenol biosynthetic process
GO:0008360 - regulation of cell shape
GO:0071555 - cell wall organization
Note(s): Note(s): ...[more].
Reference(s): [1] Dodbele S., et al., 2014
External database links:  
DIP:
 DIP-48251N
DISPROT:
 DP00516
ECOCYC:
 UPPSYN-MONOMER
ECOLIWIKI:
 b0174
INTERPRO:
 IPR001441
INTERPRO:
 IPR018520
INTERPRO:
 IPR036424
MODBASE:
 P60472
PANTHER:
 PTHR10291
PDB:
 5ZHE
PDB:
 2E9A
PDB:
 4H3A
PDB:
 5CQJ
PDB:
 5CQB
PDB:
 1JP3
PDB:
 1UEH
PDB:
 1V7U
PDB:
 1X06
PDB:
 1X07
PDB:
 1X08
PDB:
 1X09
PDB:
 2E98
PDB:
 2E99
PDB:
 4H3C
PDB:
 2E9C
PDB:
 2E9D
PDB:
 3QAS
PDB:
 3SGV
PDB:
 3SGX
PDB:
 3SH0
PDB:
 3TH8
PDB:
 3WYJ
PDB:
 4H2J
PDB:
 4H2M
PDB:
 4H2O
PDB:
 4H38
PFAM:
 PF01255
PRIDE:
 P60472
PRODB:
 PRO_000024186
PROSITE:
 PS01066
REFSEQ:
 NP_414716
SMR:
 P60472
UNIPROT:
 P60472


Operon      
Name: dxr-ispZU-cdsA         
Operon arrangement:
Transcription unit        Promoter
uppS-cdsA
uppS-cdsA
cdsA
dxr-uppS-cdsA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_334 195472 forward nd [RS-EPT-CBR] [2]
  promoter TSS_335 (cluster) 195478 forward nd [RS-EPT-CBR] [2]
  promoter TSS_336 195482 forward nd [RS-EPT-CBR] [2]
  promoter TSS_337 195485 forward nd [RS-EPT-CBR] [2]
  promoter cdsAp3 195555 forward nd [ICWHO], [RS-EPT-CBR] [2], [3]
  promoter cdsAp2 195561 forward nd [ICWHO] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Dodbele S., Martinez CD., Troutman JM., 2014, Species differences in alternative substrate utilization by the antibacterial target undecaprenyl pyrophosphate synthase., Biochemistry 53(30):5042-50

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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