RegulonDB RegulonDB 11.1: Gene Form
   

pqiA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

uup pqiB pqiA pqiC SoxS MarA pqiAp3 pqiAp3 pqiAp1 pqiAp1 pqiAp2 pqiAp2 pqiAp pqiAp

Gene      
Name: pqiA    Texpresso search in the literature
Synonym(s): ECK0941, G6490, b0950, pqi5, pqi5A
Genome position(nucleotides): 1012001 --> 1013254
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.23
External database links:  
ASAP:
 ABE-0003219
ECHOBASE:
 EB2794
ECOLIHUB:
 pqiA
OU-MICROARRAY:
 b0950
STRING:
 511145.b0950
COLOMBOS: pqiA


Product      
Name: intermembrane transport protein PqiA
Synonym(s): PqiA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 46.464
Isoelectric point: 7.907
Motif(s):
 
Type Positions Sequence Comment
49 -> 200 QRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTFFLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFVKLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDI
51 -> 71 PTAYALAALFMLLLSNLFPFV UniProt: Helical.
102 -> 122 FFLLFVQLVPAFCLITILLLV UniProt: Helical.
150 -> 170 IFLAGVLVSFVKLMAYGSIGV Topology analysis indicates that this hydrophobic region is located in the cytoplasm and does not cross the inner membrane |CITS: [27795327]|
253 -> 408 LQWTLALLVTSIMLYLPANILPIMVTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHGKRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIMTMFSAMTFDPRLSWDR

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
biological_process GO:0120009 - intermembrane lipid transfer
GO:0006950 - response to stress
GO:0061024 - membrane organization
Note(s): Note(s): ...[more].
Evidence: [EXP-IEP] Inferred from expression pattern
[EXP-IGI] Inferred from genetic interaction
Reference(s): [1] Ekiert DC., et al., 2017
[2] Isom GL., et al., 2020
[3] Koh YS., et al., 1995
[4] Nakayama T., et al., 2017
External database links:  
ALPHAFOLD:
 P0AFL9
ECOCYC:
 G6490-MONOMER
ECOLIWIKI:
 b0950
INTERPRO:
 IPR005219
INTERPRO:
 IPR007498
PFAM:
 PF04403
PRIDE:
 P0AFL9
PRODB:
 PRO_000023585
REFSEQ:
 NP_415470
SMR:
 P0AFL9
UNIPROT:
 P0AFL9


Operon      
Name: pqiABC         
Operon arrangement:
Transcription unit        Promoter
pqiABC
pqiABC
pqiABC
pqiABC
ymbA


Transcriptional Regulation      
Display Regulation             
Activated by: MarA, SoxS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Ekiert DC., Bhabha G., Isom GL., Greenan G., Ovchinnikov S., Henderson IR., Cox JS., Vale RD., 2017, Architectures of Lipid Transport Systems for the Bacterial Outer Membrane., Cell 169(2):273-285.e17

 [2] Isom GL., Coudray N., MacRae MR., McManus CT., Ekiert DC., Bhabha G., 2020, LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope., Cell 181(3):653-664.e19

 [3] Koh YS., Roe JH., 1995, Isolation of a novel paraquat-inducible (pqi) gene regulated by the soxRS locus in Escherichia coli., J Bacteriol 177(10):2673-8

 [4] Nakayama T., Zhang-Akiyama QM., 2017, pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity., J Bacteriol 199(1)

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