RegulonDB RegulonDB 10.9: Gene Form
   

nagK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lolE nagK cobB TSS_1455 TSS_1455 nagKp3 nagKp3 TSS_1454 TSS_1454 TSS_1453 TSS_1453 TSS_1452 TSS_1452

Gene      
Name: nagK    Texpresso search in the literature
Synonym(s): ECK1105, G6576, b1119, ycfX
Genome position(nucleotides): 1178593 --> 1179504 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.74
External database links:  
ASAP:
ABE-0003778
ECHOBASE:
EB3216
ECOLIHUB:
nagK
OU-MICROARRAY:
b1119
STRING:
511145.b1119
COLOMBOS: nagK


Product      
Name: N-acetyl-D-glucosamine kinase
Synonym(s): NagK, YcfX
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.043
Isoelectric point: 5.156
Motif(s):
 
Type Positions Sequence
4 -> 11 GFDIGGTK
133 -> 140 GVGGGLIF
2 -> 300 YYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVCELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRLDNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRLPVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPVARVPRIERARHGDAGGMRGAAFLH

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.34 - murein turnover, recycling
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0045127 - N-acetylglucosamine kinase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0009254 - peptidoglycan turnover
GO:0006044 - N-acetylglucosamine metabolic process
GO:0016310 - phosphorylation
GO:0046835 - carbohydrate phosphorylation
Note(s): Note(s): ...[more].
Reference(s): [1] Toba FA., et al., 2011
External database links:  
DIP:
DIP-11548N
ECOCYC:
G6576-MONOMER
ECOLIWIKI:
b1119
INTERPRO:
IPR000600
INTERPRO:
IPR043129
INTERPRO:
IPR023505
MODBASE:
P75959
PANTHER:
PTHR18964
PFAM:
PF00480
PRIDE:
P75959
PROSITE:
PS01125
REFSEQ:
NP_415637
SMR:
P75959
SWISSMODEL:
P75959
UNIPROT:
P75959


Operon      
Name: nagK-cobB         
Operon arrangement:
Transcription unit        Promoter
nagK-cobB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1452 1177616 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1453 1178231 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1454 1178238 forward nd [RS-EPT-CBR] [2]
  promoter nagKp3 1178540 forward Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter TSS_1455 1179908 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Toba FA., Thompson MG., Campbell BR., Junker LM., Rueggeberg KG., Hay AG., 2011, Role of DLP12 lysis genes in Escherichia coli biofilm formation., Microbiology 157(Pt 6):1640-50

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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