RegulonDB RegulonDB 11.1: Gene Form
   

hchA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hprS yedS hchA rseX hchAp1 hchAp1 hchAp2 hchAp2 TSS_2278 TSS_2278 rseXp rseXp

Gene      
Name: hchA    Texpresso search in the literature
Synonym(s): ECK1963, G7055, b1967, yedU, yzzC
Genome position(nucleotides): 2035835 --> 2036686
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 48.59
External database links:  
ASAP:
 ABE-0006529
ECHOBASE:
 EB1705
ECOLIHUB:
 hchA
OU-MICROARRAY:
 b1967
STRING:
 511145.b1967
COLOMBOS: hchA


Product      
Name: protein/nucleic acid deglycase 1
Synonym(s): HchA, Hsp31, Maillard deglycase 1, YedU, YzzC, glyoxalase III
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.19
Isoelectric point: 5.944
Motif(s):
 
Type Positions Sequence Comment
2 -> 283 TVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQEMLAAYAG UniProt: Protein/nucleic acid deglycase 1.
77 -> 77 E UniProt: Loss of glyoxalase activity..
185 -> 185 C UniProt: Loss of glyoxalase and aminopeptidase activities..
186 -> 186 H UniProt: Shows approximately 17% remaining glyoxalase activity compared with that of the wild-type..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
  5 - cell processes --> 5.6 - protection --> 5.6.2 - detoxification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0016790 - thiolester hydrolase activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0016811 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
GO:0008270 - zinc ion binding
GO:0042803 - protein homodimerization activity
GO:0019172 - glyoxalase III activity
GO:0036524 - protein deglycase activity
biological_process GO:0106044 - guanine deglycation
GO:0006281 - DNA repair
GO:0006974 - cellular response to DNA damage stimulus
GO:0030091 - protein repair
GO:0019243 - methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione
GO:0051595 - response to methylglyoxal
GO:0042245 - RNA repair
GO:0010447 - response to acidic pH
GO:0019249 - lactate biosynthetic process
GO:0036525 - protein deglycation
Note(s): Note(s): ...[more].
Reference(s): [1] Kim OG., et al., 2002
External database links:  
ALPHAFOLD:
 P31658
DIP:
 DIP-11851N
ECOCYC:
 G7055-MONOMER
ECOLIWIKI:
 b1967
INTERPRO:
 IPR029062
INTERPRO:
 IPR017283
MODBASE:
 P31658
PANTHER:
 PTHR43824:SF8
PDB:
 1PV2
PDB:
 1ONS
PDB:
 1N57
PDB:
 1IZY
PDB:
 1IZZ
PRIDE:
 P31658
PRODB:
 PRO_000022864
REFSEQ:
 NP_416476
SMR:
 P31658
UNIPROT:
 P31658


Operon      
Name: hchA         
Operon arrangement:
Transcription unit        Promoter
hchA
hchA


Transcriptional Regulation      
Display Regulation             
Repressed by: H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2278 2035198 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Kim OG., Kim IK., Kim GH., Ko J., Park C., Suh PG., Kang SO., Lee HS., Cha SS., 2002, Crystallization and preliminary X-ray crystallographic analysis of a yedU gene product from Escherichia coli., Acta Crystallogr D Biol Crystallogr 58(Pt 7):1217-9

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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