RegulonDB

Gene
Name:	ytfE
Synonym(s):	ECK4205, G7866, b4209, dnrN
Genome position(nucleotides):	4431321 <-- 4431983
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.09
External database links:

ASAP:	ABE-0013766
ECHOBASE:	EB2398
ECOLIHUB:	ytfE
OU-MICROARRAY:	b4209
STRING:	511145.b4209
COLOMBOS:	ytfE

Product

Name:

iron-sulfur cluster repair protein YtfE

Synonym(s):

DnrN, RIC, YtfE, protein involved in repair of stress-damaged iron-sulfur clusters

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

24.883

Isoelectric point:

5.041

Motif(s):

Type	Positions	Sequence	Comment
	5 -> 58	DQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARKELDVEVIEAELAKL
	77 -> 216	DHIIVRYHDRHREQLPELILQATKVERVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEHDEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRA

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0046872 - metal ion binding GO:0005506 - iron ion binding
	biological_process	GO:0006979 - response to oxidative stress GO:0030091 - protein repair GO:0051409 - response to nitrosative stress GO:0018283 - iron incorporation into metallo-sulfur cluster

Note(s):

Note(s): ...[more].

Evidence:

[EXP-IDA-PURIFIED-PROTEIN] Assay of protein purified to homogeneity
[EXP-IMP] Inferred from mutant phenotype

Reference(s):

[1] Alvarez-Carreno C., et al., 2018
[2] Justino MC., et al., 2006
[3] Justino MC., et al., 2007
[4] Justino MC., et al., 2005
[5] Rodionov DA., et al., 2005

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

ytfE

Operon arrangement:

Transcription unit	Promoter
ytfE	ytfEp

Transcriptional Regulation
	Display Regulation
Activated by:	NarL, NarP
Repressed by:	NsrR, FNR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_5068	4430493		forward	nd	[RS-EPT-CBR]	[6]
	promoter	TSS_5069	4431082		forward	nd	[RS-EPT-CBR]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Alvarez-Carreno C., Alva V., Becerra A., Lazcano A., 2018, Structure, function and evolution of the hemerythrin-like domain superfamily., Protein Sci 27(4):848-860
[2] Justino MC., Almeida CC., Goncalves VL., Teixeira M., Saraiva LM., 2006, Escherichia coli YtfE is a di-iron protein with an important function in assembly of iron-sulphur clusters., FEMS Microbiol Lett 257(2):278-84
[3] Justino MC., Almeida CC., Teixeira M., Saraiva LM., 2007, Escherichia coli di-iron YtfE protein is necessary for the repair of stress-damaged iron-sulfur clusters., J Biol Chem 282(14):10352-9
[4] Justino MC., Vicente JB., Teixeira M., Saraiva LM., 2005, New genes implicated in the protection of anaerobically grown Escherichia coli against nitric oxide., J Biol Chem 280(4):2636-43
[5] Rodionov DA., Dubchak IL., Arkin AP., Alm EJ., Gelfand MS., 2005, Dissimilatory metabolism of nitrogen oxides in bacteria: comparative reconstruction of transcriptional networks., PLoS Comput Biol 1(5):e55
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.