RegulonDB RegulonDB 10.9: Gene Form
   

aceB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aceB aceA metA IclR CRP IclR IclR IHF IclR IclR IclR IclR IHF Cra terminator TSS_4837 TSS_4837 TSS_4836 TSS_4836 TSS_4835 TSS_4835 aceBp aceBp

Gene      
Name: aceB    Texpresso search in the literature
Synonym(s): ECK4006, EG10023, b4014, mas
Genome position(nucleotides): 4215478 --> 4217079 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.75
Reference(s): [1] Byrne C., et al., 1988
[2] Byrne C., et al., 1988
External database links:  
ASAP:
 ABE-0013125
CGSC:
 1051
ECHOBASE:
 EB0022
ECOLIHUB:
 aceB
OU-MICROARRAY:
 b4014
STRING:
 511145.b4014
COLOMBOS: aceB


Product      
Name: malate synthase A
Synonym(s): AceB, Mas
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 60.274
Isoelectric point: 5.283
Motif(s):
 
Type Positions Sequence
12 -> 532 AFTRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKMVINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLL

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.2 - glyoxylate bypass
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0004474 - malate synthase activity
biological_process GO:0006097 - glyoxylate cycle
GO:0006099 - tricarboxylic acid cycle
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
 MALATE-SYNTHASE
ECOLIWIKI:
 b4014
INTERPRO:
 IPR011076
INTERPRO:
 IPR006252
INTERPRO:
 IPR019830
INTERPRO:
 IPR001465
MODBASE:
 P08997
PANTHER:
 PTHR42902
PDB:
 3CV2
PDB:
 3CV1
PDB:
 3CUZ
PFAM:
 PF01274
PRIDE:
 P08997
PRODB:
 PRO_000022037
PROSITE:
 PS00510
REFSEQ:
 NP_418438
SMR:
 P08997
UNIPROT:
 P08997


Operon      
Name: aceBAK         
Operon arrangement:
Transcription unit        Promoter
aceBAK
aceK


Transcriptional Regulation      
Display Regulation             
Activated by: IHF, Cra
Repressed by: CRP, IclR, ArcA
Growth Conditions:

[1] 

C: Escherichia coli| ihfB knockout mutant| minimal medium| glucose 2%| mid exponential phase
E: Escherichia coli| ihfB knockout mutant| minimal medium| sodium acetate 2%| mid exponential phase

[2] 

C: Escherichia coli| ihfB knockout mutant| minimal medium| sodium acetate 2%| mid exponential phase
E: Escherichia coli| iclR knockout mutant; ihfB knockout mutant| minimal medium| sodium acetate 2%| mid exponential phase

[3] 

C: Escherichia coli| minimal medium| glucose 2%| mid exponential phase
E: Escherichia coli| minimal medium| sodium acetate 2%| mid exponential phase


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4835 4215404 forward nd [RS-EPT-CBR] [3]
  promoter TSS_4836 4217043 forward nd [RS-EPT-CBR] [3]
  promoter TSS_4837 4217361 forward nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Byrne C., Stokes HW., Ward KA., 1988, Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli., Nucleic Acids Res 16(19):9342

 [2] Byrne C., Stokes HW., Ward KA., 1988, Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli., Nucleic Acids Res 16(22):10924

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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