RegulonDB RegulonDB 10.9: Gene Form
   

ackA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

pta ackA yfbV anti-anti-terminator terminator anti-terminator TSS_2612 TSS_2612 TSS_2611 TSS_2611 TSS_2610 TSS_2610 TSS_2609 TSS_2609 TSS_2608 TSS_2608 TSS_2607 TSS_2607 TSS_2606 (cluster) TSS_2606 (cluster) TSS_2605 TSS_2605 TSS_2604 TSS_2604 ptap ptap TSS_2602 TSS_2602 TSS_2601 TSS_2601 TSS_2600 TSS_2600 TSS_2599 TSS_2599 TSS_2598 TSS_2598 TSS_2597 TSS_2597 TSS_2596 TSS_2596 TSS_2595 TSS_2595 TSS_2594 TSS_2594 TSS_2593 TSS_2593 TSS_2592 (cluster) TSS_2592 (cluster) ackAp ackAp TSS_2591 (cluster) TSS_2591 (cluster) TSS_2590 (cluster) TSS_2590 (cluster) TSS_2589 (cluster) TSS_2589 (cluster) TSS_2588 (cluster) TSS_2588 (cluster) TSS_2587 (cluster) TSS_2587 (cluster) TSS_2586 TSS_2586 TSS_2585 TSS_2585

Gene      
Name: ackA    Texpresso search in the literature
Synonym(s): ECK2290, EG10027, b2296
Genome position(nucleotides): 2413470 --> 2414672 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.2
Reference(s): [1] Matsuyama A., et al., 1989
External database links:  
ASAP:
ABE-0007579
CGSC:
1048
ECHOBASE:
EB0026
ECOLIHUB:
ackA
OU-MICROARRAY:
b2296
STRING:
511145.b2296
COLOMBOS: ackA


Product      
Name: acetate kinase
Synonym(s): AckA, acetate kinase A, propionate kinase 2
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 43.29
Isoelectric point: 6.237
Motif(s):
 
Type Positions Sequence
221 -> 221 R
240 -> 240 M
285 -> 287 DCR
5 -> 393 LVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARIKWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSSVVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEESYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVSAIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGLLGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGIGENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIAQ
210 -> 214 HLGNG

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.3 - amino acids
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.28 - pyruvate oxidation
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.29 - acetate catabolism
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.30 - threonine catabolism
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0016774 - phosphotransferase activity, carboxyl group as acceptor
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0008776 - acetate kinase activity
GO:0000287 - magnesium ion binding
biological_process GO:0006082 - organic acid metabolic process
GO:0006083 - acetate metabolic process
GO:0006085 - acetyl-CoA biosynthetic process
GO:0016310 - phosphorylation
GO:0044011 - single-species biofilm formation on inanimate substrate
GO:0019413 - acetate biosynthetic process
GO:0019542 - propionate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-9042N
ECOCYC:
ACETATEKINA-MONOMER
ECOLIWIKI:
b2296
INTERPRO:
IPR023865
INTERPRO:
IPR043129
INTERPRO:
IPR000890
INTERPRO:
IPR004372
MODBASE:
P0A6A3
PANTHER:
PTHR21060
PDB:
1LRG
PFAM:
PF00871
PRIDE:
P0A6A3
PRINTS:
PR00471
PRODB:
PRO_000022041
PROSITE:
PS01075
PROSITE:
PS01076
REFSEQ:
NP_416799
SMR:
P0A6A3
UNIPROT:
P0A6A3


Operon      
Name: ackA-pta         
Operon arrangement:
Transcription unit        Promoter
ackA
pta
ackA-pta


Transcriptional Regulation      
Display Regulation             
Activated by: FNR, ArcA


Regulation by small RNA    
  Display Regulation
small RNA sdhX


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2585 2412788 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2586 2413305 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2587 (cluster) 2413344 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2588 (cluster) 2413425 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2589 (cluster) 2413437 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2590 (cluster) 2413451 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2591 (cluster) 2413455 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2592 (cluster) 2413462 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2593 2413470 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2594 2413474 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2595 2413477 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2596 2413482 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2597 2413792 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2598 2413797 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2599 2413805 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2600 2413957 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2601 2414269 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2602 2414360 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2604 2415098 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2605 2415351 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2606 (cluster) 2415354 forward For this promoter, there
Read more >
[RS-EPT-CBR] [2]
  promoter TSS_2607 2415750 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2608 2415781 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2609 2415814 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2610 2415818 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2611 2416038 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2612 2416149 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Matsuyama A., Yamamoto H., Nakano E., 1989, Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene., J Bacteriol 171(1):577-80

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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