RegulonDB

Gene
Name:	ampE
Synonym(s):	ECK0110, EG10042, b0111
Genome position(nucleotides):	119281 --> 120135 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.05
External database links:

ASAP:	ABE-0000382
CGSC:	30481
ECHOBASE:	EB0040
OU-MICROARRAY:	b0111
PortEco:	ampE
STRING:	511145.b0111
COLOMBOS:	ampE

Product

Name:

protein AmpE

Synonym(s):

AmpE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

32.122

Isoelectric point:

10.058

Motif(s):

Type	Positions	Sequence
	44 -> 60	LGMTIIAMGVTFLLLRA
	184 -> 198	RLQSGIDAVLHVLDW
	261 -> 264	SMAK
	74 -> 87	WLLIGLLCIGAGKV
	1 -> 284	MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTLGMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRNDSHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPVTLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKALPAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIALLTIYGALV

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:0046677 - response to antibiotic

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

ampDE

Operon arrangement:

Transcription unit	Promoter
ampDE	ampD

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_232	118078	reverse	nd	[RS-EPT-CBR]	[1]
promoter	ampDp5	118681	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 5.285. P-value: 9.708e-5 Read more >	[ICWHO]	[2]
promoter	TSS_233	120916	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_234 (cluster)	120919	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 120918 to 120919, where the highest frequency is at position 120919. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[1]
promoter	TSS_235	120955	reverse	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78