RegulonDB

Gene
Name:	ampE
Synonym(s):	ECK0110, EG10042, b0111
Genome position(nucleotides):	119281 --> 120135
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.05
External database links:

ASAP:	ABE-0000382
CGSC:	30481
ECHOBASE:	EB0040
ECOLIHUB:	ampE
OU-MICROARRAY:	b0111
STRING:	511145.b0111
COLOMBOS:	ampE

Product

Name:

protein AmpE

Synonym(s):

AmpE

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

32.122

Isoelectric point:

10.058

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 284	MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTLGMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRNDSHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPVTLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKALPAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIALLTIYGALV
	44 -> 60	LGMTIIAMGVTFLLLRA	UniProt: Helical.
	68 -> 84	VPTLLVWLLIGLLCIGA	UniProt: Helical.
	74 -> 87	WLLIGLLCIGAGKV	similarity to Walker A motif
	99 -> 101	SRN

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:0046677 - response to antibiotic

Note(s):

Note(s): ...[more].

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODB:

REFSEQ:

UNIPROT:

Operon

Name:

ampDE

Operon arrangement:

Transcription unit	Promoter
ampDE	ampD

Transcriptional Regulation
	Display Regulation
Repressed by:	CRP

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_232	118078	reverse	nd	[RS-EPT-CBR]	[1]
promoter	ampDp5	118681	forward	nd	[COMP-AINF]	[2]
promoter	TSS_233	120916	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_234 (cluster)	120919	reverse	nd	[RS-EPT-CBR]	[1]
promoter	TSS_235	120955	reverse	nd	[RS-EPT-CBR]	[1]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78