RegulonDB RegulonDB 10.9: Gene Form
   

apt gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dnaX apt ybaN Cis-reg; frameshift_element TSS_636 TSS_636 TSS_635 (cluster) TSS_635 (cluster) ybaBp ybaBp TSS_634 TSS_634 TSS_633 TSS_633 dnaXp dnaXp aptp aptp

Gene      
Name: apt    Texpresso search in the literature
Synonym(s): ECK0463, EG10051, b0469
Genome position(nucleotides): 491412 --> 491963 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.35
Reference(s): [1] Hershey HV., et al., 1986
External database links:  
ASAP:
ABE-0001628
CGSC:
1029
ECHOBASE:
EB0049
ECOLIHUB:
apt
MIM:
614723
OU-MICROARRAY:
b0469
STRING:
511145.b0469
COLOMBOS: apt


Product      
Name: adenine phosphoribosyltransferase
Synonym(s): Apt
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 19.859
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence
32 -> 156 TSLLEDPKAYALSIDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDLEYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFII

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.33 - nucleotide and nucleoside conversions
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0016757 - transferase activity, transferring glycosyl groups
GO:0016740 - transferase activity
GO:0003999 - adenine phosphoribosyltransferase activity
GO:0000287 - magnesium ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009116 - nucleoside metabolic process
GO:0006166 - purine ribonucleoside salvage
GO:0006168 - adenine salvage
GO:0044209 - AMP salvage
Note(s): Note(s): ...[more].
Reference(s): [2] Arthur PK., et al., 2011
[3] Benson CE., et al., 1970
[4] Burton K. 1977
[5] Chen KS., et al., 1993
[6] Flower AM., et al., 1991
[7] Hershey HV., et al., 1982
[8] Hershey HV., et al., 1986
[1] Hershey HV., et al., 1986
[9] Hill DL., et al., 1973
[10] Hochstadt J. 1978
[11] Hochstadt J., et al., 1976
[12] Iarulin VR., et al., 1985
[13] Khemiri A., et al., 2014
[14] Kocharian ShM. 1977
[15] Kocharian ShM., et al., 1977
[16] Kocharian ShM., et al., 1982
[17] Kocharian ShM., et al., 1975
[18] Kocharian ShM., et al., 1977
[19] Kocharian ShM., et al., 1976
[20] Levine RA., et al., 1979
[21] Levine RA., et al., 1981
[22] Livshits VA. 1976
[23] Naylor SL., et al., 1977
[24] Nygaard P. 1977
[25] Page MG., et al., 1978
[26] Zhang Y., et al., 2018
External database links:  
DIP:
DIP-36165N
ECOCYC:
ADENPRIBOSYLTRAN-MONOMER
ECOLIWIKI:
b0469
INTERPRO:
IPR000836
INTERPRO:
IPR029057
INTERPRO:
IPR005764
MODBASE:
P69503
PDB:
2DY0
PFAM:
PF00156
PRIDE:
P69503
PROSITE:
PS00103
REFSEQ:
NP_415002
SMR:
P69503
SWISSMODEL:
P69503
UNIPROT:
P69503


Operon      
Name: apt         
Operon arrangement:
Transcription unit        Promoter
apt


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_633 493064 forward nd [RS-EPT-CBR] [27]
  promoter TSS_634 493071 forward nd [RS-EPT-CBR] [27]
  promoter TSS_635 (cluster) 493804 forward For this promoter, there
Read more >
[RS-EPT-CBR] [27]
  promoter TSS_636 494018 forward nd [RS-EPT-CBR] [27]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Hershey HV., Taylor MW., 1986, Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes., Gene 43(3):287-93

 [2] Arthur PK., Alvarado LJ., Dayie TK., 2011, Expression, purification and analysis of the activity of enzymes from the pentose phosphate pathway., Protein Expr Purif 76(2):229-37

 [3] Benson CE., Love SH., Remy CN., 1970, Inhibition of de novo purine biosynthesis and interconversion by 6-methylpurine in Escherichia coli., J Bacteriol 101(3):872-80

 [4] Burton K., 1977, Transport of adenine, hypoxanthine and uracil into Escherichia coli., Biochem J 168(2):195-204

 [5] Chen KS., Saxena P., Walker JR., 1993, Expression of the Escherichia coli dnaX gene., J Bacteriol 175(20):6663-70

 [6] Flower AM., McHenry CS., 1991, Transcriptional organization of the Escherichia coli dnaX gene., J Mol Biol 220(3):649-58

 [7] Hershey HV., Gutstein R., Taylor MW., 1982, Cloning and restriction map of the E. coli apt gene., Gene 19(1):89-92

 [8] Hershey HV., Taylor MW., 1986, Sequence of the E. coli APRT gene., Adv Exp Med Biol 195 Pt A:239-46

 [9] Hill DL., Pittillo RF., 1973, Use of Escherichia coli mutants to evaluate purines, purine nucleosides, and analogues., Antimicrob Agents Chemother 4(2):125-32

 [10] Hochstadt J., 1978, Adenine phosphoribosyltransferase from Escherichia coli., Methods Enzymol 51:558-67

 [11] Hochstadt J., Quinlan D., 1976, The function and activity of certain membrane enzymes when localized on- and off- the membrane., J Cell Physiol 89(4):839-52

 [12] Iarulin VR., Gorlenko ZhM., 1985, [Effect of mutation changes in RNA-polymerase and transcription termination factor rho on expression of various operons in E. coli]., Mol Gen Mikrobiol Virusol (10):8-19

 [13] Khemiri A., Carriere M., Bremond N., Ben Mlouka MA., Coquet L., Llorens I., Chapon V., Jouenne T., Cosette P., Berthomieu C., 2014, Escherichia coli response to uranyl exposure at low pH and associated protein regulations., PLoS One 9(2):e89863

 [14] Kocharian ShM., 1977, [Genetic control of Escherichia coli K-12 strains' assimilation of 2,6-diaminopurine as a purine source]., Genetika 13(7):1252-9

 [15] Kocharian ShM., Chukanova TI., Sukhodolets VV., 1977, [Mutations of resistance to 2,6-diaminopurine and 6-methylpurine that affect adenine phosphoribosyltransferase in Escherichia coli K-12]., Genetika 13(10):1821-30

 [16] Kocharian ShM., Kocharian AM., Meliksetian GO., Akopian ZhI., 1982, [Escherichia coli K-12 mutants assimilating adenine via a new metabolic pathway]., Genetika 18(6):906-15

 [17] Kocharian ShM., Livshits VA., Sukhodolets VV., 1975, [Genetic study of Escherichia coli K-12 mutants resistant to 2,6-diaminopurine]., Genetika 11(11):79-89

 [18] Kocharian ShM., Smirnov IuV., 1977, [Escherichia coli K-12 mutants capable of catabolizing purine nucleosides in the absence of purine nucleoside phosphorylase]., Genetika 13(8):1425-33

 [19] Kocharian ShM., sukhodolets VV., 1976, [Phenotypic manifestation of mutations involving resistance to 2,6-diaminopurine (apt) in the genome of purine auxotrophs of Escherichia coli K-12], Genetika 12(7):100-8

 [20] Levine RA., Taylor MW., 1979, Regulation of purine savage enzymes in E. coli., Adv Exp Med Biol 122B:57-60

 [21] Levine RA., Taylor MW., 1981, Selection for purine regulatory mutants in an E. coli hypoxanthine phosphoribosyl transferase-guanine phosphoribosyl transferase double mutant., Mol Gen Genet 181(3):313-8

 [22] Livshits VA., 1976, [Effect of 2,6-diaminopurine resistance mutations on adenine and adenosine assimilation by cells of an adenine-dependent strain of Escherichia coli K-12]., Genetika 12(7):180-2

 [23] Naylor SL., Klebe RJ., 1977, Bioautography: a general method for the visualization of isozymes., Biochem Genet 15(11-12):1193-211

 [24] Nygaard P., 1977, Functioning of purine salvage pathways in Escherichia coli K-12., Adv Exp Med Biol 76A:186-95

 [25] Page MG., Burton K., 1978, The location of purine phosphoribosyltransferase activities in Escherichia coli., Biochem J 174(3):717-25

 [26] Zhang Y., Zbornikova E., Rejman D., Gerdes K., 2018, Novel (p)ppGpp Binding and Metabolizing Proteins of Escherichia coli., MBio 9(2)

 [27] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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