RegulonDB RegulonDB 11.1: Gene Form
   

aroA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aroA serC ycaL terminator anti-terminator anti-anti-terminator terminator anti-terminator anti-anti-terminator ycaLp6 ycaLp6 ycaLp9 ycaLp9 ycaLp8 ycaLp8 ycaLp1 ycaLp1 TSS_1177 (cluster) TSS_1177 (cluster) TSS_1176 TSS_1176 TSS_1175 TSS_1175 TSS_1174 TSS_1174 TSS_1173 TSS_1173 TSS_1172 TSS_1172

Gene      
Name: aroA    Texpresso search in the literature
Synonym(s): ECK0899, EG10073, b0908
Genome position(nucleotides): 958812 --> 960095
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.78
External database links:  
ASAP:
 ABE-0003095
CGSC:
 1008
ECHOBASE:
 EB0071
ECOLIHUB:
 aroA
OU-MICROARRAY:
 b0908
STRING:
 511145.b0908
COLOMBOS: aroA


Product      
Name: 3-phosphoshikimate 1-carboxyvinyltransferase
Synonym(s): AroA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 46.096
Isoelectric point: 5.27
Motif(s):
 
Type Positions Sequence Comment
7 -> 420 QPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNLLDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPLAAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGGNVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQHYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRFADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNIYNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQL
22 -> 23 KS UniProt: Shikimate-3-phosphate binding; Sequence Annotation Type: region of interest.
23 -> 23 S UniProt: In Ref. 1; CAA25223..
94 -> 97 NAGT UniProt: Phosphoenolpyruvate; Sequence Annotation Type: region of interest.
96 -> 96 G UniProt: Insensitive to glyphosate with unaltered affinity for its first substrate S3P, but displays a 30-fold lower affinity for its second substrate PEP..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.20 - chorismate
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0016740 - transferase activity
GO:0003866 - 3-phosphoshikimate 1-carboxyvinyltransferase activity
biological_process GO:0008652 - cellular amino acid biosynthetic process
GO:0009073 - aromatic amino acid family biosynthetic process
GO:0009423 - chorismate biosynthetic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A6D3
DIP:
 DIP-48256N
ECOCYC:
 AROA-MONOMER
ECOLIWIKI:
 b0908
INTERPRO:
 IPR006264
INTERPRO:
 IPR036968
INTERPRO:
 IPR001986
INTERPRO:
 IPR013792
INTERPRO:
 IPR023193
MODBASE:
 P0A6D3
PDB:
 2AA9
PDB:
 2AAY
PDB:
 2PQ9
PDB:
 2QFQ
PDB:
 2QFS
PDB:
 2QFT
PDB:
 2QFU
PDB:
 3FJX
PDB:
 3FJZ
PDB:
 3FK0
PDB:
 3FK1
PDB:
 1X8T
PDB:
 1X8R
PDB:
 1Q36
PDB:
 1Q0J
PDB:
 1Q0I
PDB:
 1P89
PDB:
 1P88
PDB:
 1MI4
PDB:
 1G6T
PDB:
 1EPS
PDB:
 1G6S
PFAM:
 PF00275
PRIDE:
 P0A6D3
PRODB:
 PRO_000022142
PROSITE:
 PS00885
PROSITE:
 PS00104
REFSEQ:
 NP_415428
SMR:
 P0A6D3
UNIPROT:
 P0A6D3


Operon      
Name: serC-aroA         
Operon arrangement:
Transcription unit        Promoter
serC
serC
serC-aroA


Transcriptional Regulation      
Display Regulation             
Activated by: Lrp
Repressed by: CRP


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1172 957945 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1173 958104 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1174 958585 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1175 958602 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1176 958749 forward nd [RS-EPT-CBR] [1]
  promoter TSS_1177 (cluster) 958751 forward nd [RS-EPT-CBR] [1]
  promoter ycaLp1 960132 forward nd [COMP-AINF] [2]
  promoter ycaLp8 960143 forward nd [COMP-AINF] [2]
  promoter ycaLp9 960238 forward nd [COMP-AINF] [2]
  promoter ycaLp6 960255 forward nd [COMP-AINF] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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