RegulonDB RegulonDB 11.1: Gene Form
   

aspA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

aspA dcuA fxsA aspX DcuR NarL CRP NarL DcuR NarL FNR anti-anti-terminator anti-terminator terminator anti-anti-terminator anti-terminator fxsAp fxsAp aspAp aspAp TSS_4948 TSS_4948 TSS_4947 TSS_4947 TSS_4946 TSS_4946 TSS_4945 TSS_4945 TSS_4944 TSS_4944 aspXp2 aspXp2 dcuAp1 dcuAp1 TSS_4943 TSS_4943 TSS_4942 TSS_4942 TSS_4941 (cluster) TSS_4941 (cluster) TSS_4940 TSS_4940 TSS_4939 TSS_4939 TSS_4938 TSS_4938 TSS_4937 TSS_4937 TSS_4936 TSS_4936 TSS_4935 TSS_4935 TSS_4934 TSS_4934

Gene      
Name: aspA    Texpresso search in the literature
Synonym(s): ECK4133, EG10095, b4139
Genome position(nucleotides): 4366891 <-- 4368327
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 49.2
Reference(s): [1] Golby P., et al., 1998
External database links:  
ASAP:
 ABE-0013551
CGSC:
 991
ECHOBASE:
 EB0093
ECOLIHUB:
 aspA
OU-MICROARRAY:
 b4139
STRING:
 511145.b4139
COLOMBOS: aspA


Product      
Name: aspartate ammonia-lyase
Synonym(s): AspA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 52.356
Isoelectric point: 4.952
Motif(s):
 
Type Positions Sequence Comment
10 -> 10 D UniProt: Loss of activity..
13 -> 345 GTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSLIKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNIQRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGAYVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIG
15 -> 15 R UniProt: No effect on Kcat. Increases KM for aspartic acid 2.5-fold..
26 -> 26 H UniProt: No effect on Kcat. Increases KM for aspartic acid 3-fold..
29 -> 29 R UniProt: No effect on Kcat. Increases KM for aspartic acid 40-fold..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.6 - asparagine
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.7 - lysine
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.13 - amino acid conversion
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0008797 - aspartate ammonia-lyase activity
GO:0042802 - identical protein binding
biological_process GO:0006538 - glutamate catabolic process
GO:0006533 - aspartate catabolic process
GO:0006099 - tricarboxylic acid cycle
GO:0006531 - aspartate metabolic process
GO:0019740 - nitrogen utilization
GO:0051289 - protein homotetramerization
Reference(s): [2] Woods SA., et al., 1986
External database links:  
ALPHAFOLD:
 P0AC38
DIP:
 DIP-36166N
ECOCYC:
 ASPARTASE-MONOMER
ECOLIWIKI:
 b4139
INTERPRO:
 IPR022761
INTERPRO:
 IPR024083
INTERPRO:
 IPR020557
INTERPRO:
 IPR018951
INTERPRO:
 IPR008948
INTERPRO:
 IPR004708
INTERPRO:
 IPR000362
MODBASE:
 P0AC38
PDB:
 1JSW
PFAM:
 PF00206
PFAM:
 PF10415
PRIDE:
 P0AC38
PRINTS:
 PR00149
PRODB:
 PRO_000022171
PROSITE:
 PS00163
REFSEQ:
 NP_418562
SMR:
 P0AC38
UNIPROT:
 P0AC38


Operon      
Name: aspA-dcuA         
Operon arrangement:
Transcription unit        Promoter
dcuA
aspA
aspA-dcuA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, FNR, DcuR
Repressed by: NarL


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4934 4366101 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4935 4366103 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4936 4366114 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4937 4366119 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4938 4366313 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4939 4366748 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4940 4366806 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4941 (cluster) 4366809 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4942 4366833 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4943 4366838 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4944 4367788 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4945 4367803 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4946 4368190 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4947 4368393 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_4948 4368431 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Golby P., Kelly DJ., Guest JR., Andrews SC., 1998, Transcriptional regulation and organization of the dcuA and dcuB genes, encoding homologous anaerobic C4-dicarboxylate transporters in Escherichia coli., J Bacteriol 180(24):6586-96

 [2] Woods SA., Miles JS., Roberts RE., Guest JR., 1986, Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12., Biochem J 237(2):547-57

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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