RegulonDB

Gene
Name:	avtA
Synonym(s):	ECK3561, EG10107, b3572
Genome position(nucleotides):	3739705 --> 3740958
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.15
External database links:

ASAP:	ABE-0011671
CGSC:	971
ECHOBASE:	EB0105
ECOLIHUB:	avtA
OU-MICROARRAY:	b3572
STRING:	511145.b3572
COLOMBOS:	avtA

Product

Name:

valine—pyruvate aminotransferase

Synonym(s):

AvtA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

46.711

Isoelectric point:

5.83

Motif(s):

Type	Positions	Sequence	Comment
	165 -> 391	FKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVIDNAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGIISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHKPEGAIFLWLWFKDLPITTKQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNY
	208 -> 208	A	UniProt: In Ref. 2; AAB18549..

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.17 - alanine
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003824 - catalytic activity GO:0016740 - transferase activity GO:0008483 - transaminase activity GO:0030170 - pyridoxal phosphate binding GO:0009042 - valine-pyruvate transaminase activity
	biological_process	GO:0009058 - biosynthetic process GO:0009099 - valine biosynthetic process GO:0042852 - L-alanine biosynthetic process GO:0030632 - D-alanine biosynthetic process GO:1901605 - alpha-amino acid metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Berg CM., et al., 1988
[2] Cho BK., et al., 2003
[3] Holtzclaw WD., et al., 1977
[4] Liu L., et al., 1987
[5] Lorenz E., et al., 2013
[6] Vartak NB., et al., 1991
[7] Wang MD., et al., 1987

External database links:

ALPHAFOLD:

P09053

ECOCYC:

VALINE-PYRUVATE-AMINOTRANSFER-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

avtA

Operon arrangement:

Transcription unit	Promoter
avtA	avtAp

Transcriptional Regulation
	Display Regulation
Repressed by:	Lrp

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Berg CM., Wang MD., Vartak NB., Liu L., 1988, Acquisition of new metabolic capabilities: multicopy suppression by cloned transaminase genes in Escherichia coli K-12., Gene 65(2):195-202
[2] Cho BK., Cho HJ., Park SH., Yun H., Kim BG., 2003, Simultaneous synthesis of enantiomerically pure (S)-amino acids and (R)-amines using coupled transaminase reactions., Biotechnol Bioeng 81(7):783-9
[3] Holtzclaw WD., Chapman LF., 1977, A new assay for transaminase C., Anal Biochem 83(1):162-7
[4] Liu L., Whalen W., Das A., Berg CM., 1987, Rapid sequencing of cloned DNA using a transposon for bidirectional priming: sequence of the Escherichia coli K-12 avtA gene., Nucleic Acids Res 15(22):9461-9
[5] Lorenz E., Klatte S., Wendisch VF., 2013, Reductive amination by recombinant Escherichia coli: whole cell biotransformation of 2-keto-3-methylvalerate to L-isoleucine., J Biotechnol 168(3):289-94
[6] Vartak NB., Liu L., Wang BM., Berg CM., 1991, A functional leuABCD operon is required for leucine synthesis by the tyrosine-repressible transaminase in Escherichia coli K-12., J Bacteriol 173(12):3864-71
[7] Wang MD., Buckley L., Berg CM., 1987, Cloning of genes that suppress an Escherichia coli K-12 alanine auxotroph when present in multicopy plasmids., J Bacteriol 169(12):5610-4