RegulonDB RegulonDB 11.1: Gene Form
   

bioB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bioA bioF bioB bioC bioD BirA terminator bioBp bioBp bioAp bioAp TSS_941 TSS_941 ybhBp2 ybhBp2

Gene      
Name: bioB    Texpresso search in the literature
Synonym(s): ECK0764, EG10118, b0775
Genome position(nucleotides): 809344 --> 810384
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 55.52
External database links:  
ASAP:
 ABE-0002644
CGSC:
 958
ECHOBASE:
 EB0116
ECOLIHUB:
 bioB
OU-MICROARRAY:
 b0775
STRING:
 511145.b0775
COLOMBOS: bioB


Product      
Name: biotin synthase
Synonym(s): BioB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 38.648
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence Comment
38 -> 256 RQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAWKNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEFYGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPESVPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPT UniProt: Radical SAM core.
50 -> 203 TGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAWKNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEFYGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRA
53 -> 53 C UniProt: Total loss of activity..
57 -> 57 C UniProt: Total loss of activity..
60 -> 60 C UniProt: Total loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.1 - biotin
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0004076 - biotin synthase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0005506 - iron ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009102 - biotin biosynthetic process
External database links:  
ALPHAFOLD:
 P12996
DIP:
 DIP-9220N
ECOCYC:
 BIOTIN-SYN-MONOMER
ECOLIWIKI:
 b0775
INTERPRO:
 IPR010722
INTERPRO:
 IPR013785
INTERPRO:
 IPR007197
INTERPRO:
 IPR002684
INTERPRO:
 IPR006638
INTERPRO:
 IPR024177
MODBASE:
 P12996
PANTHER:
 PTHR22976
PDB:
 1R30
PFAM:
 PF06968
PFAM:
 PF04055
PRIDE:
 P12996
PRODB:
 PRO_000022222
PROSITE:
 PS51918
REFSEQ:
 NP_415296
SMART:
 SM00876
SMART:
 SM00729
SMR:
 P12996
UNIPROT:
 P12996


Operon      
Name: bioBFCD         
Operon arrangement:
Transcription unit        Promoter
bioBFCD


Transcriptional Regulation      
Display Regulation             
Repressed by: BirA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybhBp2 808035 reverse nd [COMP-AINF] [1]
  promoter TSS_941 809290 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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