RegulonDB RegulonDB 11.1: Gene Form
   

bioD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

uvrB bioF bioC bioD ybhU bioB DnaA LexA DnaA DnaA DnaA LexA LexA DnaA DnaA LexA DnaA uvrBp1 uvrBp1 uvrBp2 uvrBp2 uvrBp3 uvrBp3

Gene      
Name: bioD    Texpresso search in the literature
Synonym(s): ECK0767, EG10120, b0778
Genome position(nucleotides): 812270 --> 812947
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.69
External database links:  
ASAP:
 ABE-0002650
CGSC:
 956
ECHOBASE:
 EB0118
ECOLIHUB:
 bioD
OU-MICROARRAY:
 b0778
STRING:
 511145.b0778
COLOMBOS: bioD


Product      
Name: dethiobiotin synthetase
Synonym(s): BioD
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 24.14
Isoelectric point: 5.639
Motif(s):
 
Type Positions Sequence Comment
2 -> 225 SKRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGSEKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAGLRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTAQVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGKYINLALL UniProt: ATP-dependent dethiobiotin synthetase BioD 1.
3 -> 208 KRYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGSEKTPEGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAGLRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTAQVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLA
12 -> 12 T UniProt: Strong decrease in ATP affinity; essential role for this residue in the steady-state affinity for ATP..
13 -> 13 E UniProt: Almost no change in activity..
16 -> 16 K UniProt: Complete loss of activity..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.1 - biotin
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0046872 - metal ion binding
GO:0016874 - ligase activity
GO:0004141 - dethiobiotin synthase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0000287 - magnesium ion binding
GO:0042803 - protein homodimerization activity
biological_process GO:0009102 - biotin biosynthetic process
External database links:  
ALPHAFOLD:
 P13000
ECOCYC:
 DETHIOBIOTIN-SYN-MONOMER
ECOLIWIKI:
 b0778
INTERPRO:
 IPR004472
INTERPRO:
 IPR027417
MODBASE:
 P13000
PANTHER:
 PTHR43210
PDB:
 1DAI
PDB:
 1DAK
PDB:
 1DAM
PDB:
 1DBS
PDB:
 1DTS
PDB:
 1DAH
PDB:
 1DAG
PDB:
 1DAF
PDB:
 1DAE
PDB:
 1DAD
PDB:
 1BYI
PDB:
 1A82
PDB:
 1BS1
PFAM:
 PF13500
PRIDE:
 P13000
PRODB:
 PRO_000022224
REFSEQ:
 NP_415299
SMR:
 P13000
UNIPROT:
 P13000


Operon      
Name: bioBFCD         
Operon arrangement:
Transcription unit        Promoter
bioBFCD


Transcriptional Regulation      
Display Regulation             
Repressed by: BirA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References



RegulonDB