RegulonDB RegulonDB 11.1: Gene Form
   

dapA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bamC dapA gcvR anti-anti-terminator anti-terminator bcpp bcpp TSS_2774 TSS_2774 TSS_2773 TSS_2773 gcvRp1 gcvRp1 gcvRp2 gcvRp2 dapAp dapAp TSS_2770 TSS_2770 TSS_2769 TSS_2769 bamCp bamCp TSS_2768 TSS_2768

Gene      
Name: dapA    Texpresso search in the literature
Synonym(s): ECK2474, EG10205, b2478
Genome position(nucleotides): 2598882 <-- 2599760
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 51.76
External database links:  
ASAP:
 ABE-0008163
CGSC:
 880
ECHOBASE:
 EB0201
ECOLIHUB:
 dapA
OU-MICROARRAY:
 b2478
STRING:
 511145.b2478
COLOMBOS: dapA


Product      
Name: 4-hydroxy-tetrahydrodipicolinate synthase
Synonym(s): DHDPS, DapA, dihydrodipicolinate synthase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 31.27
Isoelectric point: 6.415
Motif(s):
 
Type Positions Sequence Comment
2 -> 288 FTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGTTGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLTVTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGIKEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMCKLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITDSGRETVRAALKH
44 -> 44 T UniProt: 8% of wild-type activity. 4-fold decrease in affinity for pyruvate, but nearly no change in that for (S)-ASA..
107 -> 107 Y UniProt: Reduced kcat by 90%..
133 -> 133 Y UniProt: Reduced kcat by 99.7%. Reduced affinity for both substrates..
138 -> 138 R UniProt: Strongly increased KM for L-aspartate 4-semialdehyde. No effect on KM for pyruvate. Reduced activity by 99.7%..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.1 - amino acids --> 1.5.1.7 - lysine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0008840 - 4-hydroxy-tetrahydrodipicolinate synthase activity
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0008652 - cellular amino acid biosynthetic process
GO:0009085 - lysine biosynthetic process
GO:0009089 - lysine biosynthetic process via diaminopimelate
GO:0019877 - diaminopimelate biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Truffa-Bachi P., et al., 1967
External database links:  
ALPHAFOLD:
 P0A6L2
ECOCYC:
 DIHYDRODIPICSYN-MONOMER
ECOLIWIKI:
 b2478
INTERPRO:
 IPR002220
INTERPRO:
 IPR020624
INTERPRO:
 IPR020625
INTERPRO:
 IPR005263
INTERPRO:
 IPR013785
MODBASE:
 P0A6L2
PANTHER:
 PTHR12128
PDB:
 3C0J
PDB:
 3DEN
PDB:
 3DU0
PDB:
 3I7Q
PDB:
 3I7R
PDB:
 3I7S
PDB:
 4EOU
PDB:
 5T25
PDB:
 5T26
PDB:
 2PUR
PDB:
 2OJP
PDB:
 2ATS
PDB:
 2A6N
PDB:
 2A6L
PDB:
 1YXD
PDB:
 1YXC
PDB:
 1S5W
PDB:
 1S5V
PDB:
 1DHP
PDB:
 1S5T
PFAM:
 PF00701
PRIDE:
 P0A6L2
PRINTS:
 PR00146
PRODB:
 PRO_000022400
PROSITE:
 PS00665
PROSITE:
 PS00666
REFSEQ:
 NP_416973
SMART:
 SM01130
SMR:
 P0A6L2
UNIPROT:
 P0A6L2


Operon      
Name: dapA-bamC         
Operon arrangement:
Transcription unit        Promoter
bamC
dapA-nlpB


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2768 2598879 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2769 2599483 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2770 2599588 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_2773 2600100 forward nd [RS-EPT-CBR] [2]
  promoter TSS_2774 2600225 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Truffa-Bachi P., Patte JC., Cohen GN., 1967, [On the dihydrodipicolinate synthetase of Escherichia coli K12]., C R Acad Sci Hebd Seances Acad Sci D 265(13):928-9

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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