RegulonDB RegulonDB 11.1: Gene Form
   

edd gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

edd zwf eda GntR KdgR Cra GntR KdgR PhoB anti-anti-terminator anti-terminator terminator TSS_2197 TSS_2197 TSS_2196 TSS_2196 eddp eddp edap1 edap1 edap2 edap2 edap3 edap3 TSS_2193 TSS_2193 TSS_2192 TSS_2192 TSS_2191 TSS_2191

Gene      
Name: edd    Texpresso search in the literature
Synonym(s): ECK1852, EG10257, b1851
Genome position(nucleotides): 1932793 <-- 1934604
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.64
Reference(s): [1] Carter AT., et al., 1993
[2] Conway T., et al., 1991
[3] Egan SE., et al., 1992
[4] Peyru G., et al., 1968
External database links:  
ASAP:
 ABE-0006166
CGSC:
 825
ECHOBASE:
 EB0253
ECOLIHUB:
 edd
OU-MICROARRAY:
 b1851
STRING:
 511145.b1851
COLOMBOS: edd


Product      
Name: phosphogluconate dehydratase
Synonym(s): Edd
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 64.639
Isoelectric point: 6.351
Motif(s):
 
Type Positions Sequence Comment
66 -> 598 NNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGA
252 -> 254 PLR UniProt: In Ref. 1; AAA23722..

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.9 - Entner-Doudoroff
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0046872 - metal ion binding
GO:0016836 - hydro-lyase activity
GO:0051536 - iron-sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0004456 - phosphogluconate dehydratase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0009255 - Entner-Doudoroff pathway through 6-phosphogluconate
GO:0019521 - D-gluconate metabolic process
GO:0046177 - D-gluconate catabolic process
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0ADF6
DIP:
 DIP-47906N
ECOCYC:
 PGLUCONDEHYDRAT-MONOMER
ECOLIWIKI:
 b1851
INTERPRO:
 IPR037237
INTERPRO:
 IPR004786
INTERPRO:
 IPR020558
INTERPRO:
 IPR000581
INTERPRO:
 IPR042096
PANTHER:
 PTHR43661:SF1
PFAM:
 PF00920
PRIDE:
 P0ADF6
PRODB:
 PRO_000022503
PROSITE:
 PS00887
PROSITE:
 PS00886
REFSEQ:
 NP_416365
SMR:
 P0ADF6
UNIPROT:
 P0ADF6


Operon      
Name: edd-eda         
Operon arrangement:
Transcription unit        Promoter
eda
eda
eda
edd-eda


Transcriptional Regulation      
Display Regulation             
Repressed by: KdgR, Cra, GntR


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2191 1932530 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2192 1932726 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2193 1932744 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2196 1935229 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2197 1935719 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Carter AT., Pearson BM., Dickinson JR., Lancashire WE., 1993, Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway., Gene 130(1):155-6

 [2] Conway T., Yi KC., Egan SE., Wolf RE., Rowley DL., 1991, Locations of the zwf, edd, and eda genes on the Escherichia coli physical map., J Bacteriol 173(17):5247-8

 [3] Egan SE., Fliege R., Tong S., Shibata A., Wolf RE., Conway T., 1992, Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon., J Bacteriol 174(14):4638-46

 [4] Peyru G., Fraenkel DG., 1968, Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli., J Bacteriol 95(4):1272-8

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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