RegulonDB

Gene
Name:	edd
Synonym(s):	ECK1852, EG10257, b1851
Genome position(nucleotides):	1932793 <-- 1934604 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.64
Reference(s):	[1] Carter AT., et al., 1993 [2] Conway T., et al., 1991 [3] Egan SE., et al., 1992 [4] Peyru G., et al., 1968
External database links:

ASAP:	ABE-0006166
CGSC:	825
ECHOBASE:	EB0253
ECOLIHUB:	edd
OU-MICROARRAY:	b1851
STRING:	511145.b1851
COLOMBOS:	edd

Product

Name:

phosphogluconate dehydratase

Synonym(s):

Edd

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

64.639

Isoelectric point:

6.351

Motif(s):

Type	Positions	Sequence
	66 -> 598	NNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVGQVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPGLTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAPGTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWMPIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMARLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGELDWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVVFESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVTDGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAAREPHIPDLSASRVGTGRELFSALREKLSGAEQGA
	252 -> 254	PLR

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.9 - Entner-Doudoroff
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003824 - catalytic activity GO:0016829 - lyase activity GO:0046872 - metal ion binding GO:0016836 - hydro-lyase activity GO:0051536 - iron-sulfur cluster binding GO:0051539 - 4 iron, 4 sulfur cluster binding GO:0004456 - phosphogluconate dehydratase activity
	biological_process	GO:0005975 - carbohydrate metabolic process GO:0009255 - Entner-Doudoroff pathway through 6-phosphogluconate GO:0019521 - D-gluconate metabolic process GO:0046177 - D-gluconate catabolic process

Note(s):

Note(s): ...[more].

External database links:

DIP:

DIP-47906N

ECOCYC:

PGLUCONDEHYDRAT-MONOMER

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

edd-eda

Operon arrangement:

Transcription unit	Promoter
eda	edap3
eda	edap2
eda	edap1
edd-eda	eddp

Transcriptional Regulation
	Display Regulation
Repressed by:	KdgR, Cra, GntR

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2191	1932530	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2192	1932726	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2193	1932744	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2196	1935229	reverse	nd	[RS-EPT-CBR]	[5]
promoter	TSS_2197	1935719	reverse	nd	[RS-EPT-CBR]	[5]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Carter AT., Pearson BM., Dickinson JR., Lancashire WE., 1993, Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway., Gene 130(1):155-6
[2] Conway T., Yi KC., Egan SE., Wolf RE., Rowley DL., 1991, Locations of the zwf, edd, and eda genes on the Escherichia coli physical map., J Bacteriol 173(17):5247-8
[3] Egan SE., Fliege R., Tong S., Shibata A., Wolf RE., Conway T., 1992, Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon., J Bacteriol 174(14):4638-46
[4] Peyru G., Fraenkel DG., 1968, Genetic mapping of loci for glucose-6-phosphate dehydrogenase, gluconate-6-phosphate dehydrogenase, and gluconate-6-phosphate dehydrase in Escherichia coli., J Bacteriol 95(4):1272-8
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.