RegulonDB

RegulonDB RegulonDB 10.10: Gene Form

entB gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	entB
Synonym(s):	ECK0588, EG10260, b0595, entG
Genome position(nucleotides):	627694 --> 628551 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.36
External database links:

ASAP:	ABE-0002052
CGSC:	821
ECHOBASE:	EB0256
ECOLIHUB:	entB
OU-MICROARRAY:	b0595
STRING:	511145.b0595
COLOMBOS:	entB

Product

Name:

enterobactin synthase component B

Synonym(s):

EntB, EntG

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,inner membrane

Molecular weight:

32.554

Isoelectric point:

4.851

Motif(s):

Type	Positions	Sequence
	242 -> 242	G
	263 -> 263	D
	264 -> 264	F
	249 -> 249	M
	209 -> 284	APIPASKAALREVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREV

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.14 - enterochelin (enterobactin)
		2 - information transfer --> 2.3 - protein related --> 2.3.9 - Non-ribosomal peptide synthetase
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol GO:0005886 - plasma membrane
	molecular_function	GO:0003824 - catalytic activity GO:0016765 - transferase activity, transferring alkyl or aryl (other than methyl) groups GO:0005515 - protein binding GO:0016787 - hydrolase activity GO:0046872 - metal ion binding GO:0016874 - ligase activity GO:0008908 - isochorismatase activity GO:0000287 - magnesium ion binding GO:0031177 - phosphopantetheine binding GO:0042802 - identical protein binding GO:0047527 - 2,3-dihydroxybenzoate-serine ligase activity
	biological_process	GO:0008152 - metabolic process GO:0009239 - enterobactin biosynthetic process

Note(s):

Note(s): ...[more].

Evidence:

[APPHINH] Assay of protein purified to homogeneity from its native host

Reference(s):

[1] Chen D., et al., 2009
[2] Gehring AM., et al., 1998
[3] Hubrich F., et al., 2013
[4] Khalil S., et al., 2009
[5] Woodrow GC., et al., 1975
[6] Zhou Z., et al., 2007

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

entCEBAH

Operon arrangement:

Transcription unit	Promoter
entCEBA-ybdB	entCp
entH	entHp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	Fur

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Chen D., Wu R., Bryan TL., Dunaway-Mariano D., 2009, In vitro kinetic analysis of substrate specificity in enterobactin biosynthetic lower pathway enzymes provides insight into the biochemical function of the hot dog-fold thioesterase EntH., Biochemistry 48(3):511-3
[2] Gehring AM., Mori I., Walsh CT., 1998, Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF., Biochemistry 37(8):2648-59
[3] Hubrich F., Mordhorst S., Andexer JN., 2013, Cinnamic acid derivatives as inhibitors for chorismatases and isochorismatases., Bioorg Med Chem Lett 23(5):1477-81
[4] Khalil S., Pawelek PD., 2009, Ligand-induced conformational rearrangements promote interaction between the Escherichia coli enterobactin biosynthetic proteins EntE and EntB., J Mol Biol 393(3):658-71
[5] Woodrow GC., Young IG., Gibson F., 1975, Mu-induced polarity in the Escherichia coli K-12 ent gene cluster: evidence for a gene (entG) involved in the biosynthesis of enterochelin., J Bacteriol 124(1):1-6
[6] Zhou Z., Lai JR., Walsh CT., 2007, Directed evolution of aryl carrier proteins in the enterobactin synthetase., Proc Natl Acad Sci U S A 104(28):11621-6

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