RegulonDB

RegulonDB RegulonDB 10.8: Gene Form

folD gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	folD
Synonym(s):	ECK0522, EG10328, ads, b0529
Genome position(nucleotides):	556875 <-- 557741 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	54.33
External database links:

ASAP:	ABE-0001818
CGSC:	31127
ECHOBASE:	EB0324
NCBI-GENE:	945221
OU-MICROARRAY:	b0529
PortEco:	folD
STRING:	511145.b0529
COLOMBOS:	folD

Product

Name:

bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Synonym(s):

Ads, FolD

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

31.044

Isoelectric point:

5.97

Motif(s):

Type	Positions	Sequence
	166 -> 168	GAS
	2 -> 288	AAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSNPASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLPAGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGLNAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPGDWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTLQACVEYHDPQDE
	124 -> 279	HPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGLNAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPGDWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTLQAC
	200 -> 200	V
	5 -> 121	IIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSNPASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLPAGIDNVKVLERIHPDKDVD

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.2 - folic acid
		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.17 - formyl-THF biosynthesis
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0004486 - methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity GO:0003824 - catalytic activity GO:0016787 - hydrolase activity GO:0016491 - oxidoreductase activity GO:0004477 - methenyltetrahydrofolate cyclohydrolase activity GO:0004488 - methylenetetrahydrofolate dehydrogenase (NADP+) activity
	biological_process	GO:0008152 - metabolic process GO:0000105 - histidine biosynthetic process GO:0006164 - purine nucleotide biosynthetic process GO:0006730 - one-carbon metabolic process GO:0008652 - cellular amino acid biosynthetic process GO:0009086 - methionine biosynthetic process GO:0055114 - oxidation-reduction process GO:0035999 - tetrahydrofolate interconversion

Note(s):

Note(s): ...[more].

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PANTHER:

PDB:

PDB:

PDB:

PDB:

PFAM:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

folD-ybcJ

Operon arrangement:

Transcription unit	Promoter
folD-ybcJ	folDp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	ybcJp4	556891	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.934. P-value: 6.144e-6 Read more >	[ICWHO]	[1]
promoter	ybcJp3	556967	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.804. P-value: 3.908e-5 Read more >	[ICWHO]	[1]
promoter	ybcJp5	557006	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.329. P-value: 2.699e-5 Read more >	[ICWHO]	[1]
promoter	ybcJp7	557025	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.746. P-value: 9.516e-5 Read more >	[ICWHO]	[1]
promoter	TSS_679	557596	reverse	nd	[RS-EPT-CBR]	[2]
promoter	sfmAp4	558062	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.973. P-value: 1.980e-5 Read more >	[ICWHO]	[1]
promoter	sfmAp6	558166	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.176. P-value: 5.275e-5 Read more >	[ICWHO]	[1]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

[2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

RegulonDB