RegulonDB RegulonDB 10.8: Gene Form
   

fruK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

Gene      
Name: fruK    Texpresso search in the literature
Synonym(s): ECK2161, EG10337, b2168, fpk
Genome position(nucleotides): 2261427 <-- 2262365 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.46
External database links:  
ASAP:
ABE-0007174
CGSC:
743
ECHOBASE:
EB0333
OU-MICROARRAY:
b2168
PortEco:
fruK
STRING:
511145.b2168
COLOMBOS: fruK


Product      
Name: 1-phosphofructokinase
Synonym(s): Fpk, FruK
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.756
Isoelectric point: 5.207
Motif(s):
 
Type Positions Sequence
14 -> 293 YDLVGFCPEIERGEVNLVKTTGLHAAGKGINVAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGEVTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQCPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIAHVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLRLATAVAALAVSQSNVGI
187 -> 190 NRRE

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016740 - transferase activity
GO:0016301 - kinase activity
GO:0016773 - phosphotransferase activity, alcohol group as acceptor
GO:0008443 - phosphofructokinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008662 - 1-phosphofructokinase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0016310 - phosphorylation
GO:0006001 - fructose catabolic process
GO:0046835 - carbohydrate phosphorylation
Note(s): Note(s): ...[more].
Reference(s): [1] Bol'shakova TN., et al., 1991
[2] Bolshakova TN., et al., 1992
[3] Dobrynina OIu., et al., 1995
[4] Molchanova ML., et al., 1992
External database links:  
ECOCYC:
1-PFK-MONOMER
ECOLIWIKI:
b2168
INTERPRO:
IPR017583
INTERPRO:
IPR029056
INTERPRO:
IPR022463
INTERPRO:
IPR011611
INTERPRO:
IPR002173
MODBASE:
P0AEW9
PFAM:
PF00294
PRIDE:
P0AEW9
PRODB:
PRO_000022701
PROSITE:
PS00584
PROSITE:
PS00583
REFSEQ:
NP_416673
SMR:
P0AEW9
UNIPROT:
P0AEW9


Operon      
Name: fruBKA         
Operon arrangement:
Transcription unit        Promoter
fruBKA


Transcriptional Regulation      
Display Regulation             
Repressed by: Cra


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2462 2260442 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2463 2262092 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2464 2262611 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_2465 2263594 reverse nd [RS-EPT-CBR] [5]
  promoter setBp2 2263713 forward Similarity to the consensus
Read more >
[ICWHO] [6]
  promoter setBp3 2263836 forward Similarity to the consensus
Read more >
[ICWHO] [6]
  promoter TSS_2466 2263839 forward nd [RS-EPT-CBR] [5]


Reference(s)    

 [1] Bol'shakova TN., Molchanova ML., Erlagaeva RS., Grigorenko IuA., Gershanovich VN., 1991, [Uncoupling expression of genes coding for the synthesis of proteins involved in transport and utilization of fructose in Escherichia coli K-12]., Genetika 27(11):1912-9

 [2] Bolshakova TN., Molchanova ML., Erlagaeva RS., Grigorenko YA., Gershanovitch VN., 1992, A novel mutation FruS, altering synthesis of components of the phosphoenolpyruvate: fructose phosphotransferase system in Escherichia coli K12., Mol Gen Genet 232(3):394-8

 [3] Dobrynina OIu., Bol'shakova TN., Umiarov AM., Gershanovich VN., 1995, [Determination of the direction of transcription of Escherichia coli K-12 structural genes of the fructose regulon in vivo], Genetika 31(2):276-7

 [4] Molchanova ML., Erdagaeva RS., Grigorenko IuA., Bol'shakova TN., Gershanovich VN., 1992, [Role of fructose-1-phosphate kinase in expression of PEP-synthase in Escherichia coli K-12]., Genetika 28(8):46-51

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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