RegulonDB RegulonDB 11.1: Gene Form
   

fumC gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fumA fumC rstB tus CRP CRP CRP MarA SoxS Rob terminator fumCp2 fumCp2 fumCp1 fumCp1 tusp2 tusp2

Gene      
Name: fumC    Texpresso search in the literature
Synonym(s): ECK1606, EG10358, b1611
Genome position(nucleotides): 1685185 <-- 1686588
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.77
External database links:  
ASAP:
 ABE-0005380
CGSC:
 15273
ECHOBASE:
 EB0353
ECOLIHUB:
 fumC
MIM:
 606812
MIM:
 136850
MIM:
 150800
OU-MICROARRAY:
 b1611
STRING:
 511145.b1611
COLOMBOS: fumC


Product      
Name: fumarase C
Synonym(s): FumC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 50.489
Isoelectric point: 6.561
Motif(s):
 
Type Positions Sequence Comment
12 -> 342 GAIDVPADKLWGAQTQRSLEHFRISTEKMPTSLIHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQSNMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQLIPQLKTLTQTLNEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYSLPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQAHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALTMLCCQVMG
98 -> 100 SGT UniProt: Substrate binding; Sequence Annotation Type: region of interest.
126 -> 126 R UniProt: 10-fold decrease of fumarase activity..
127 -> 127 K UniProt: No effect..
129 -> 132 HPND UniProt: Substrate binding (B site); Sequence Annotation Type: region of interest.

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0045239 - tricarboxylic acid cycle enzyme complex
molecular_function GO:0003824 - catalytic activity
GO:0016829 - lyase activity
GO:0004333 - fumarate hydratase activity
GO:0042802 - identical protein binding
biological_process GO:0008152 - metabolic process
GO:0006099 - tricarboxylic acid cycle
GO:0006979 - response to oxidative stress
GO:0006108 - malate metabolic process
GO:0006106 - fumarate metabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Calderon IL., et al., 2009
[2] Chen YP., et al., 2012
[3] Guest JR., et al., 1985
[4] Woods SA., et al., 1986
External database links:  
ALPHAFOLD:
 P05042
DIP:
 DIP-9719N
ECOCYC:
 FUMC-MONOMER
ECOLIWIKI:
 b1611
INTERPRO:
 IPR024083
INTERPRO:
 IPR005677
INTERPRO:
 IPR000362
INTERPRO:
 IPR008948
INTERPRO:
 IPR022761
INTERPRO:
 IPR018951
INTERPRO:
 IPR020557
MODBASE:
 P05042
PANTHER:
 PTHR11444
PDB:
 6P3C
PDB:
 6OS7
PDB:
 6NZC
PDB:
 6NZB
PDB:
 6NZA
PDB:
 6NZ9
PDB:
 2FUS
PDB:
 1YFE
PDB:
 1KQ7
PDB:
 1FUR
PDB:
 1FUQ
PDB:
 1FUO
PDB:
 1FUP
PFAM:
 PF00206
PFAM:
 PF10415
PRIDE:
 P05042
PRINTS:
 PR00149
PRODB:
 PRO_000022736
PROSITE:
 PS00163
REFSEQ:
 NP_416128
SMR:
 P05042
UNIPROT:
 P05042


Operon      
Name: fumAC         
Operon arrangement:
Transcription unit        Promoter
fumC
fumC
fumA
fumAC


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, SoxR, MarA, Rob, SoxS
Repressed by: FNR, Fur, ArcA
Undetermined regulation CRP


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter tusp2 1684173 forward nd [COMP-AINF] [5]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight


Reference(s)    

 [1] Calderon IL., Elias AO., Fuentes EL., Pradenas GA., Castro ME., Arenas FA., Perez JM., Vasquez CC., 2009, Tellurite-mediated disabling of [4Fe-4S] clusters of Escherichia coli dehydratases., Microbiology 155(Pt 6):1840-6

 [2] Chen YP., Lin HH., Yang CD., Huang SH., Tseng CP., 2012, Regulatory role of cAMP receptor protein over Escherichia coli fumarase genes., J Microbiol 50(3):426-33

 [3] Guest JR., Miles JS., Roberts RE., Woods SA., 1985, The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC)., J Gen Microbiol 131(11):2971-84

 [4] Woods SA., Miles JS., Roberts RE., Guest JR., 1986, Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12., Biochem J 237(2):547-57

 [5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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