RegulonDB

Gene
Name:	galR
Synonym(s):	ECK2835, EG10364, Rgal, b2837
Genome position(nucleotides):	2976599 --> 2977630 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.71
External database links:

ASAP:	ABE-0009307
CGSC:	721
ECHOBASE:	EB0359
OU-MICROARRAY:	b2837
PortEco:	galR
STRING:	511145.b2837
COLOMBOS:	galR

Product

Name:

DNA-binding transcriptional dual regulator GalR

Synonym(s):

GalR

Sequence:

Get amino acid sequence Fasta Format

Regulator Family:

GalR/LacI

Cellular location:

cytosol

Molecular weight:

37.094

Isoelectric point:

6.237

Motif(s):

Type	Positions	Sequence
	4 -> 23	IKDVARLAGVSVATVSRVIN
	168 -> 329	TRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGEPDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVLVSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSV
	1 -> 56	MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPNANARALAQ
	3 -> 48	TIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLSYHPN

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
		2 - information transfer --> 2.2 - RNA related --> 2.2.2 - Transcription related
		3 - regulation --> 3.1 - type of regulation --> 3.1.2 - transcriptional level --> 3.1.2.3 - repressor
		3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0003677 - DNA binding GO:0005515 - protein binding GO:0003700 - DNA-binding transcription factor activity GO:0042802 - identical protein binding GO:0000976 - transcription regulatory region sequence-specific DNA binding
	biological_process	GO:0005975 - carbohydrate metabolic process GO:0006012 - galactose metabolic process GO:0006351 - transcription, DNA-templated GO:0006355 - regulation of transcription, DNA-templated

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype
[IPI] Inferred from physical interaction

Reference(s):

[1] Aki T., et al., 1997
[2] Choy HE., et al., 1997
[3] Hsieh M., et al., 1994
[4] Roy S., et al., 2005
[5] Roy S., et al., 2004
[6] Semsey S., et al., 2002
[7] Semsey S., et al., 2007
[8] Stragier P., et al., 1983
[9] Weickert MJ., et al., 1993
[10] von Wilcken-Bergmann B., et al., 1982

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PANTHER:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

galR

Operon arrangement:

Transcription unit	Promoter
galR	galRp

Transcriptional Regulation
	Display Regulation
Repressed by:	GalS, GalR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3139	2977006		forward	nd	[RS-EPT-CBR]	[11]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Aki T., Adhya S., 1997, Repressor induced site-specific binding of HU for transcriptional regulation., EMBO J 16(12):3666-74
[2] Choy HE., Hanger RR., Aki T., Mahoney M., Murakami K., Ishihama A., Adhya S., 1997, Repression and activation of promoter-bound RNA polymerase activity by Gal repressor., J Mol Biol 272(3):293-300
[3] Hsieh M., Hensley P., Brenowitz M., Fetrow JS., 1994, A molecular model of the inducer binding domain of the galactose repressor of Escherichia coli., J Biol Chem 269(19):13825-35
[4] Roy S., Dimitriadis EK., Kar S., Geanacopoulos M., Lewis MS., Adhya S., 2005, Gal repressor-operator-HU ternary complex: pathway of repressosome formation., Biochemistry 44(14):5373-80
[5] Roy S., Semsey S., Liu M., Gussin GN., Adhya S., 2004, GalR represses galP1 by inhibiting the rate-determining open complex formation through RNA polymerase contact: a GalR negative control mutant., J Mol Biol 344(3):609-18
[6] Semsey S., Geanacopoulos M., Lewis DE., Adhya S., 2002, Operator-bound GalR dimers close DNA loops by direct interaction: tetramerization and inducer binding., EMBO J 21(16):4349-56
[7] Semsey S., Krishna S., Sneppen K., Adhya S., 2007, Signal integration in the galactose network of Escherichia coli., Mol Microbiol 65(2):465-76
[8] Stragier P., Danos O., Patte JC., 1983, Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. II. Nucleotide sequence of the lysA gene and its regulatory region., J Mol Biol 168(2):321-31
[9] Weickert MJ., Adhya S., 1993, The galactose regulon of Escherichia coli., Mol Microbiol 10(2):245-51
[10] von Wilcken-Bergmann B., Muller-Hill B., 1982, Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli., Proc Natl Acad Sci U S A 79(8):2427-31
[11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.