RegulonDB RegulonDB 11.1: Gene Form
   

mnmG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mnmG rsmG mioC ppGpp TSS_4348 TSS_4348 TSS_4347 TSS_4347 mnmGp2 mnmGp2 mnmGp mnmGp TSS_4346 TSS_4346 TSS_4345 TSS_4345 rsmGp rsmGp

Gene      
Name: mnmG    Texpresso search in the literature
Synonym(s): ECK3734, EG10375, b3741, gidA, trmF
Genome position(nucleotides): 3923744 <-- 3925633
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 54.39
External database links:  
ASAP:
 ABE-0012231
CGSC:
 18277
ECHOBASE:
 EB0370
ECOLIHUB:
 mnmG
MIM:
 614702
OU-MICROARRAY:
 b3741
STRING:
 511145.b3741
COLOMBOS: mnmG


Product      
Name: 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG
Synonym(s): GidA, MnmG, TrmF, protein involved in modification of U34 in tRNA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 69.521
Isoelectric point: 6.605
Motif(s):
 
Type Positions Sequence Comment
8 -> 398 DVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADK
13 -> 18 GGGHAG UniProt: FAD.
15 -> 15 G UniProt: Decrease in FAD binding and partial loss of activity. Loss of activity; when associated with A-13..
273 -> 287 GPRYCPSIEDKVMRF UniProt: NAD.
403 -> 616 PARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEIGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREASGEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILL

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0002144 - cytosolic tRNA wobble base thiouridylase complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0050660 - flavin adenine dinucleotide binding
GO:0042803 - protein homodimerization activity
biological_process GO:0140018 - regulation of cytoplasmic translational fidelity
GO:0002926 - tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation
GO:0030488 - tRNA methylation
GO:0008033 - tRNA processing
GO:0009411 - response to UV
GO:0002098 - tRNA wobble uridine modification
Note(s): Note(s): ...[more].
Reference(s): [1] Borgese N., et al., 2010
[2] Erova TE., et al., 2012
[3] Lee C., et al., 2016
[4] Sargentini NJ., et al., 2016
External database links:  
ALPHAFOLD:
 P0A6U3
DIP:
 DIP-35786N
ECOCYC:
 EG10375-MONOMER
ECOLIWIKI:
 b3741
INTERPRO:
 IPR036188
INTERPRO:
 IPR040131
INTERPRO:
 IPR026904
INTERPRO:
 IPR020595
INTERPRO:
 IPR004416
INTERPRO:
 IPR002218
MODBASE:
 P0A6U3
PANTHER:
 PTHR11806:SF0
PANTHER:
 PTHR11806
PDB:
 3CES
PDB:
 3CP2
PFAM:
 PF13932
PFAM:
 PF01134
PRIDE:
 P0A6U3
PRODB:
 PRO_000023257
PROSITE:
 PS01280
PROSITE:
 PS01281
REFSEQ:
 NP_418197
SMR:
 P0A6U3
UNIPROT:
 P0A6U3


Operon      
Name: asnC-mioC-mnmG-rsmG         
Operon arrangement:
Transcription unit        Promoter
mnmG-rsmG
mnmG-rsmG
mioC
asnC
asnC-mioC
asnC-mioC-mnmG
rsmG


Transcriptional Regulation      
Display Regulation             
Repressed by: Nac, AsnC


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter rsmGp 3923756 reverse nd [COMP-AINF-POSITIONAL-IDENTIFICATION] [5]
  promoter TSS_4345 3924313 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_4346 3924964 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_4347 3925679 reverse nd [RS-EPT-CBR] [6]
  promoter TSS_4348 3926009 reverse nd [RS-EPT-CBR] [6]


Evidence    

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Borgese N., Righi M., 2010, Remote origins of tail-anchored proteins., Traffic 11(7):877-85

 [2] Erova TE., Kosykh VG., Sha J., Chopra AK., 2012, DNA adenine methyltransferase (Dam) controls the expression of the cytotoxic enterotoxin (act) gene of Aeromonas hydrophila via tRNA modifying enzyme-glucose-inhibited division protein (GidA)., Gene 498(2):280-7

 [3] Lee C., Kwon M., Lee K., Min H., Kim SH., Lee N., Kim J., Kim D., Ko C., Park C., 2016, Screening for Escherichia coli K-12 genes conferring glyoxal resistance or sensitivity by transposon insertions., FEMS Microbiol Lett 363(18)

 [4] Sargentini NJ., Gularte NP., Hudman DA., 2016, Screen for genes involved in radiation survival of Escherichia coli and construction of a reference database., Mutat Res 793-794:1-14

 [5] Benitez-Paez A., Villarroya M., Armengod ME., 2012, Regulation of expression and catalytic activity of Escherichia coli RsmG methyltransferase., RNA 18(4):795-806

 [6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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