RegulonDB RegulonDB 11.1: Gene Form
   

rsmG gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mnmG rsmG atpI TSS_4346 TSS_4346 TSS_4345 TSS_4345 rsmGp rsmGp atpIp atpIp TSS_4344 TSS_4344 atpBp1 atpBp1 atpBp2 atpBp2 TSS_4343 TSS_4343 TSS_4342 (cluster) TSS_4342 (cluster) TSS_4341 (cluster) TSS_4341 (cluster)

Gene      
Name: rsmG    Texpresso search in the literature
Synonym(s): ECK3733, EG10376, b3740, gidB
Genome position(nucleotides): 3923057 <-- 3923680
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 48.72
External database links:  
ASAP:
 ABE-0012229
CGSC:
 18274
ECHOBASE:
 EB0371
ECOLIHUB:
 rsmG
OU-MICROARRAY:
 b3740
STRING:
 511145.b3740
COLOMBOS: rsmG


Product      
Name: 16S rRNA m7G527 methyltransferase
Synonym(s): GidB, RsmG, rRNA small subunit 7-methylguanosine (m7G) methyltransferase, ribosomal RNA small subunit methyltransferase G
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 23.431
Isoelectric point: 6.517
Motif(s):
 
Type Positions Sequence Comment
20 -> 200 QKNQLIAYVNMLHKWNKAYNLTSVRDPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRVRFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGRFYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLV
39 -> 39 N UniProt: Lack of activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity..
53 -> 53 H UniProt: Decrease in activity. Very low-level streptomycin resistance. Slight decrease in AdoMet affinity..
56 -> 56 D UniProt: Strong decrease in activity. Low-level streptomycin resistance. Slight decrease in AdoMet affinity..
71 -> 71 D UniProt: Lack of activity. Low-level streptomycin resistance. 10-fold increase in AdoMet affinity..

 
Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0008168 - methyltransferase activity
GO:0016740 - transferase activity
GO:0008649 - rRNA methyltransferase activity
GO:0070043 - rRNA (guanine-N7-)-methyltransferase activity
biological_process GO:0006364 - rRNA processing
GO:0032259 - methylation
GO:0070475 - rRNA base methylation
GO:0070476 - rRNA (guanine-N7)-methylation
Note(s): Note(s): ...[more].
External database links:  
ALPHAFOLD:
 P0A6U5
DIP:
 DIP-47992N
ECOCYC:
 EG10376-MONOMER
ECOLIWIKI:
 b3740
INTERPRO:
 IPR003682
INTERPRO:
 IPR029063
MODBASE:
 P0A6U5
PANTHER:
 PTHR31760
PDB:
 1JSX
PFAM:
 PF02527
PRIDE:
 P0A6U5
PRODB:
 PRO_000023887
REFSEQ:
 NP_418196
SMR:
 P0A6U5
UNIPROT:
 P0A6U5


Operon      
Name: asnC-mioC-mnmG-rsmG         
Operon arrangement:
Transcription unit        Promoter
mnmG-rsmG
mnmG-rsmG
mioC
asnC
asnC-mioC
asnC-mioC-mnmG
rsmG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4341 (cluster) 3922078 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4342 (cluster) 3922116 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4343 3922124 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4344 3922273 reverse nd [RS-EPT-CBR] [1]
  promoter rsmGp 3923756 reverse nd [COMP-AINF-POSITIONAL-IDENTIFICATION] [2]
  promoter TSS_4345 3924313 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_4346 3924964 reverse nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [COMP-AINF-POSITIONAL-IDENTIFICATION] Automated inference of promoter position


Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Benitez-Paez A., Villarroya M., Armengod ME., 2012, Regulation of expression and catalytic activity of Escherichia coli RsmG methyltransferase., RNA 18(4):795-806

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