RegulonDB

Gene
Name:	glgC
Synonym(s):	ECK3416, EG10379, b3430
Genome position(nucleotides):	3568033 <-- 3569328
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.62
External database links:

ASAP:	ABE-0011204
CGSC:	708
ECHOBASE:	EB0374
ECOLIHUB:	glgC
OU-MICROARRAY:	b3430
STRING:	511145.b3430
COLOMBOS:	glgC

Gene

Name:

glgC

Synonym(s):

ECK3416, EG10379, b3430

Genome position(nucleotides):

3568033 <-- 3569328

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

52.62

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

2 -> 431

VSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPAVHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLLPAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLVSGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPEGMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER

UniProt: Glucose-1-phosphate adenylyltransferase.

22 -> 291

ALILAGGRGTRLKDLTNKRAKPAVHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLLPAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKGARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVFDADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRDVGTLEAYWKANLDLA

39 -> 39

UniProt: The level of activation by pyridoxal phosphate and fructose-1,6-phosphate is only approximately 2-fold compared to activation of 15- to 28-fold respectively, for the wild-type. NADPH is unable to activate the mutant enzyme..

44 -> 44

UniProt: In SG14 mutant; lower apparent affinities for substrates, fructose-1,6-bisphosphate and AMP..

67 -> 67

UniProt: In CL1136 mutant; less dependent on the allosteric activator, fructose-1,6-bisphosphate, for activity and less sensitive to inhibition by AMP..

Type

Positions

Sequence

Comment

2 -> 431

UniProt: Glucose-1-phosphate adenylyltransferase.

22 -> 291

39 -> 39

44 -> 44

UniProt: In SG14 mutant; lower apparent affinities for substrates, fructose-1,6-bisphosphate and AMP..

67 -> 67

UniProt: In CL1136 mutant; less dependent on the allosteric activator, fructose-1,6-bisphosphate, for activity and less sensitive to inhibition by AMP..

74 -> 74

UniProt: Insensitive to activation by fructose-1,6-bisphosphate, but still binds fructose-1,6-bisphosphate with similar affinity as the wild-type. AMP causes similar inhibition on the wild-type and mutant..

113 -> 113

UniProt: The enzyme is activated 15-fold by fructose-1,6-bisphosphate..

114 -> 114

UniProt: Shows a decrease of affinity for the substrates and less than 2-fold activation by fructose 1,6-bisphosphate in the ADP-glucose synthesis direction. In contrast, in the pyrophosphorolysis direction, the mutant shoqws about a 30-fold activation by fructose 1,6-bisphosphate..

161 -> 161

UniProt: In Ref. 3; CAA23544/AAA98736/AAA23873..

166 -> 166

UniProt: In Ref. 3; CAA23544/AAA98736/AAA23873..

189 -> 189

UniProt: In Ref. 3; CAA23544/AAA98736/AAA23873..

194 -> 195

UniProt: Alpha-D-glucose 1-phosphate binding; Sequence Annotation Type: region of interest.

195 -> 195

UniProt: 600-fold decrease of the affinity for alpha-D-glucose 1-phosphate compared to the wild-type. In contrast, kinetic constants for ATP, magnesium and fructose-1,6-bisphosphate are similar in mutant and wild-type cases. The catalytic efficiency is 2-fold lower in the mutant..

295 -> 295

UniProt: In SG5 mutant..

296 -> 296

UniProt: In Ref. 3; CAA23544/AAA98736..

336 -> 336

UniProt: In 618 mutant; causes lowered affinity for AMP..

419 -> 423

REMLR

UniProt: Fructose-1,6-bisphosphate binding; Sequence Annotation Type: region of interest.

429 -> 431

QER

UniProt: Fructose-1,6-bisphosphate binding; Sequence Annotation Type: region of interest.

Multifun Terms (GenProtEC)

1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.9 - cytoplasmic polysaccharides

Gene Ontology Terms (GO)

cellular_component

GO:0010170 - glucose-1-phosphate adenylyltransferase complex
GO:0005829 - cytosol

molecular_function

GO:0003824 - catalytic activity
GO:0016779 - nucleotidyltransferase activity
GO:0016740 - transferase activity
GO:0008878 - glucose-1-phosphate adenylyltransferase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0000287 - magnesium ion binding
GO:0016208 - AMP binding
GO:0042802 - identical protein binding

biological_process

GO:0008152 - metabolic process
GO:0005975 - carbohydrate metabolic process
GO:0009058 - biosynthetic process
GO:0005977 - glycogen metabolic process
GO:0005978 - glycogen biosynthetic process
GO:0051289 - protein homotetramerization

Transcriptional Regulation
	Display Regulation
Activated by:	CRP, PhoP

Transcriptional Regulation

Display Regulation

Activated by:

CRP, PhoP

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_4027	3564324	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4028	3565337	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4029	3565453	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4030	3566562	reverse	nd	[RS-EPT-CBR]	[4]
promoter	glgPp1	3566773	reverse	nd	[COMP-AINF]	[5]
promoter	TSS_4031	3567478	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4032	3567563	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4033	3567895	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4034	3568396	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4035	3568634	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4036	3568661	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4037	3568712	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4038	3568714	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4039	3569225	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4040 (cluster)	3569457	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4041	3570215	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4042	3570251	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_4043	3570750	reverse	nd	[RS-EPT-CBR]	[4]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_4027

3564324

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4028

3565337

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4029

3565453

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4030

3566562

reverse

[RS-EPT-CBR]

[4]

promoter

glgPp1

3566773

reverse

[COMP-AINF]

[5]

promoter

TSS_4031

3567478

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4032

3567563

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4033

3567895

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4034

3568396

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4035

3568634

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4036

3568661

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4037

3568712

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4038

3568714

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4039

3569225

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4040 (cluster)

3569457

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4041

3570215

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4042

3570251

reverse

[RS-EPT-CBR]

[4]

promoter

TSS_4043

3570750

reverse

[RS-EPT-CBR]

[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Adamson DN., Lim HN., 2013, Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation., Proc Natl Acad Sci U S A 110(32):13120-5
[2] Almagro G., Viale AM., Montero M., Rahimpour M., Munoz FJ., Baroja-Fernandez E., Bahaji A., Zuniga M., Gonzalez-Candelas F., Pozueta-Romero J., 2015, Comparative genomic and phylogenetic analyses of Gammaproteobacterial glg genes traced the origin of the Escherichia coli glycogen glgBXCAP operon to the last common ancestor of the sister orders Enterobacteriales and Pasteurellales., PLoS One 10(1):e0115516
[3] Diez MD., Ebrecht AC., Martinez LI., Aleanzi MC., Guerrero SA., Ballicora MA., Iglesias AA., 2013, A Chimeric UDP-glucose pyrophosphorylase produced by protein engineering exhibits sensitivity to allosteric regulators., Int J Mol Sci 14(5):9703-21
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Adamson DN., Lim HN., 2013, Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation., Proc Natl Acad Sci U S A 110(32):13120-5

[2] Almagro G., Viale AM., Montero M., Rahimpour M., Munoz FJ., Baroja-Fernandez E., Bahaji A., Zuniga M., Gonzalez-Candelas F., Pozueta-Romero J., 2015, Comparative genomic and phylogenetic analyses of Gammaproteobacterial glg genes traced the origin of the Escherichia coli glycogen glgBXCAP operon to the last common ancestor of the sister orders Enterobacteriales and Pasteurellales., PLoS One 10(1):e0115516

[3] Diez MD., Ebrecht AC., Martinez LI., Aleanzi MC., Guerrero SA., Ballicora MA., Iglesias AA., 2013, A Chimeric UDP-glucose pyrophosphorylase produced by protein engineering exhibits sensitivity to allosteric regulators., Int J Mol Sci 14(5):9703-21

[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[5] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78