RegulonDB RegulonDB 10.8: Gene Form
   

gltA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gltA ybgD ybgV ybgU terminator TSS_839 TSS_839 gltAp2 gltAp2 ybgDp9 ybgDp9

Gene      
Name: gltA    Texpresso search in the literature
Synonym(s): ECK0709, EG10402, b0720, gluT, icdB
Genome position(nucleotides): 753185 <-- 754468 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.7
External database links:  
ASAP:
ABE-0002451
CGSC:
687
ECHOBASE:
EB0397
OU-MICROARRAY:
b0720
PortEco:
gltA
STRING:
511145.b0720
COLOMBOS: gltA


Product      
Name: citrate synthase
Synonym(s): GltA, GluT, IcdB
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 48.015
Isoelectric point: 6.678
Motif(s):
 
Type Positions Sequence
46 -> 410 GFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYDEFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREIAAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILERAMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKMLEEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKDDLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGWIAHWSEMHSDGMKIARPR
289 -> 289 V
11 -> 11 N
208 -> 208 E
207 -> 207 C

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.4 - TCA cycle
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046912 - transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
GO:0004108 - citrate (Si)-synthase activity
GO:0042802 - identical protein binding
GO:0070404 - NADH binding
biological_process GO:0008152 - metabolic process
GO:0006099 - tricarboxylic acid cycle
GO:0034214 - protein hexamerization
External database links:  
DIP:
DIP-36204N
ECOCYC:
CITSYN-MONOMER
ECOLIWIKI:
b0720
INTERPRO:
IPR016143
INTERPRO:
IPR002020
INTERPRO:
IPR010953
INTERPRO:
IPR016142
INTERPRO:
IPR019810
INTERPRO:
IPR024176
INTERPRO:
IPR036969
MINT:
MINT-1249502
MODBASE:
P0ABH7
PANTHER:
PTHR11739
PDB:
4JAG
PDB:
4JAE
PDB:
4JAD
PDB:
4G6B
PDB:
1OWC
PDB:
1OWB
PDB:
1NXG
PDB:
1NXE
PDB:
4JAF
PFAM:
PF00285
PRIDE:
P0ABH7
PRINTS:
PR00143
PRODB:
PRO_000022803
PROSITE:
PS00480
REFSEQ:
NP_415248
SMR:
P0ABH7
UNIPROT:
P0ABH7


Operon      
Name: gltA         
Operon arrangement:
Transcription unit        Promoter
gltA
gltA


Transcriptional Regulation      
Display Regulation             
Activated by: CRP, IHF
Repressed by: ArcA


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter ybgDp9 752958 reverse Similarity to the consensus
Read more >
[ICWHO] [1]
  promoter TSS_839 754683 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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