RegulonDB

Gene
Name:	grpE
Synonym(s):	ECK2610, EG10416, b2614
Genome position(nucleotides):	2750115 <-- 2750708
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.34
External database links:

ASAP:	ABE-0008598
CGSC:	660
ECHOBASE:	EB0411
ECOLIHUB:	grpE
OU-MICROARRAY:	b2614
STRING:	511145.b2614
COLOMBOS:	grpE

Gene

Name:

grpE

Synonym(s):

ECK2610, EG10416, b2614

Genome position(nucleotides):

2750115 <-- 2750708

Strand:

reverse

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

50.34

External database links:

ASAP:

CGSC:

ECHOBASE:

ECOLIHUB:

OU-MICROARRAY:

STRING:

COLOMBOS:

Type

Positions

Sequence

Comment

1 -> 40

MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPR

UniProt: Disordered; Sequence Annotation Type: region of interest.

2 -> 33

SSKEQKTPEGQAPEEIIMDQHEEIEAVEPEAS

flexible N-terminal domain1

16 -> 40

EIIMDQHEEIEAVEPEASAEQVDPR

UniProt: Basic and acidic residues; Sequence Annotation Type: compositionally biased region.

35 -> 194

EQVDPRDEKVANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAK

40 -> 109

RDEKVANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVA

present in the monomer that is distal to DnaK in a GrpE-DnaK complex

Type

Positions

Sequence

Comment

1 -> 40

MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPR

UniProt: Disordered; Sequence Annotation Type: region of interest.

2 -> 33

SSKEQKTPEGQAPEEIIMDQHEEIEAVEPEAS

flexible N-terminal domain1

16 -> 40

EIIMDQHEEIEAVEPEASAEQVDPR

UniProt: Basic and acidic residues; Sequence Annotation Type: compositionally biased region.

35 -> 194

EQVDPRDEKVANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAK

40 -> 109

RDEKVANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEVA

present in the monomer that is distal to DnaK in a GrpE-DnaK complex

53 -> 53

alternate sequence E → G: sensitive to bacteriophage λ at 30°C; resistant to bacteriophage λ at 42°C

73 -> 73

UniProt: Great decrease in ability to interact with DnaK..

74 -> 74

UniProt: Great decrease in ability to interact with DnaK..

82 -> 82

UniProt: Great decrease in ability to interact with DnaK..

86 -> 86

UniProt: No effect in ability to interact with DnaK..

89 -> 109

EKFINELLPVIDSLDRALEVA

present in the monomer that is proximal to DnaK in a GrpE-DnaK complex

102 -> 102

alternate sequence L → P: sensitive to bacteriophage λ at 30°C; resistant to bacteriophage λ at 42°C

104 -> 104

UniProt: No effect in ability to interact with DnaK..

107 -> 107

UniProt: No effect in ability to interact with DnaK..

108 -> 108

UniProt: No effect in ability to interact with DnaK..

117 -> 137

SAMVEGIELTLKSMLDVVRKF

present in both monomers of the GrpE dimer in complex with DnaK ATPase domain

122 -> 122

alternate sequence G → D: resistant to bacteriophage λ at 30°C and 42°C; loss of ability to stimulate DnaK's ATPase and to interact with DnaK

127 -> 127

alternate sequence L → P: resistant to bacteriophage λ at 30°C and 42°C; loss of ability to stimulate DnaK's ATPase and to interact with DnaK

131 -> 131

alternate sequence L → P: sensitive to bacteriophage λ at 30°C; resistant to bacteriophage λ at 42°C

139 -> 197

VEVIAETNVPLDPNVHQAIAMVESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA

C-terminal β domain

149 -> 149

UniProt: No effect in ability to interact with DnaK..

151 -> 151

UniProt: No effect in ability to interact with DnaK..

154 -> 154

alternate sequence H → R: sensitive to bacteriophage λ at 30°C; resistant to bacteriophage λ at 42°C

155 -> 155

UniProt: No effect in ability to interact with DnaK..

157 -> 157

UniProt: No effect in ability to interact with DnaK..

159 -> 159

UniProt: No effect in ability to interact with DnaK..

174 -> 174

UniProt: No effect in ability to interact with DnaK..

177 -> 177

alternate sequence G → S: sensitive to bacteriophage λ at 30°C; resistant to bacteriophage λ at 42°C; unable to stimulate DnaK's ATPase at 43°C

183 -> 183

UniProt: Loss of ability to interact with DnaK..

186 -> 186

UniProt: No effect in ability to interact with DnaK..

189 -> 189

UniProt: No effect in ability to interact with DnaK..

192 -> 192

UniProt: Loss of ability to interact with DnaK..

Multifun Terms (GenProtEC)

2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)

5 - cell processes --> 5.1 - cell division

8 - extrachromosomal --> 8.1 - prophage genes and phage related functions

Gene Ontology Terms (GO)

cellular_component

GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0032991 - protein-containing complex

molecular_function

GO:0019904 - protein domain specific binding
GO:0005515 - protein binding
GO:0051082 - unfolded protein binding
GO:0000774 - adenyl-nucleotide exchange factor activity
GO:0042803 - protein homodimerization activity
GO:0051087 - chaperone binding

biological_process

GO:0006457 - protein folding
GO:0009408 - response to heat
GO:0065003 - protein-containing complex assembly
GO:0051085 - chaperone cofactor-dependent protein refolding
GO:0043335 - protein unfolding

Transcriptional Regulation
	Display Regulation
Activated by:	CRP

Transcriptional Regulation

Display Regulation

Activated by:

CRP

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_2962	2749213		forward	nd	[RS-EPT-CBR]	[1]
	promoter	nadKp2	2750763		forward	nd	[COMP-AINF]	[2]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_2962

2749213

forward

[RS-EPT-CBR]

[1]

promoter

nadKp2

2750763

forward

[COMP-AINF]

[2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[COMP-AINF] Inferred computationally without human oversight

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

[COMP-AINF] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78