RegulonDB RegulonDB 10.9: Gene Form
   

hemL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

fhuB hemL yadX fhuD PdhR PhoP terminator anti-terminator TSS_296 TSS_296 clcAp10 clcAp10 TSS_295 TSS_295 TSS_294 (cluster) TSS_294 (cluster) TSS_293 (cluster) TSS_293 (cluster) hemLp2 hemLp2 TSS_292 (cluster) TSS_292 (cluster) hemLp hemLp hemLp1 hemLp1 TSS_291 TSS_291 TSS_290 TSS_290

Gene      
Name: hemL    Texpresso search in the literature
Synonym(s): ECK0153, EG10432, b0154, gsa, popC
Genome position(nucleotides): 173602 <-- 174882 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.5
External database links:  
ASAP:
ABE-0000529
CGSC:
372
ECHOBASE:
EB0427
ECOLIHUB:
hemL
OU-MICROARRAY:
b0154
STRING:
511145.b0154
COLOMBOS: hemL


Product      
Name: glutamate-1-semialdehyde 2,1-aminomutase
Synonym(s): Gsa, HemL, PopC
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 45.366
Isoelectric point: 4.469
Motif(s):
 
Type Positions Sequence
9 -> 9 S
2 -> 2 S
32 -> 396 TPLFIEKADGAYLYDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKYTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPS
265 -> 265 K

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers --> 1.5.3.12 - heme, porphyrine
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016853 - isomerase activity
GO:0008483 - transaminase activity
GO:0030170 - pyridoxal phosphate binding
GO:0042286 - glutamate-1-semialdehyde 2,1-aminomutase activity
GO:0042803 - protein homodimerization activity
biological_process GO:0006779 - porphyrin-containing compound biosynthetic process
GO:0033014 - tetrapyrrole biosynthetic process
GO:0006782 - protoporphyrinogen IX biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Aiguo Z., et al., 2019
[2] Cui Z., et al., 2019
[3] Eguchi Y., et al., 2004
[4] Grimm B., et al., 1991
[5] Ikemi M., et al., 1992
[6] Kang Z., et al., 2011
[7] O'Neill GP., et al., 1989
[8] Zhang J., et al., 2015
[9] Zhang J., et al., 2016
[10] Zhang J., et al., 2019
External database links:  
DIP:
DIP-9886N
ECOCYC:
GSAAMINOTRANS-MONOMER
ECOLIWIKI:
b0154
INTERPRO:
IPR005814
INTERPRO:
IPR015424
INTERPRO:
IPR015422
INTERPRO:
IPR015421
INTERPRO:
IPR004639
MODBASE:
P23893
PFAM:
PF00202
PRIDE:
P23893
PRODB:
PRO_000022878
PROSITE:
PS00600
REFSEQ:
NP_414696
SMR:
P23893
SWISSMODEL:
P23893
UNIPROT:
P23893


Operon      
Name: hemL         
Operon arrangement:
Transcription unit        Promoter
hemL
hemL
hemL


Transcriptional Regulation      
Display Regulation             
Activated by: PhoP
Repressed by: PdhR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_290 173420 forward nd [RS-EPT-CBR] [11]
  promoter TSS_291 173769 reverse nd [RS-EPT-CBR] [11]
  promoter TSS_292 (cluster) 174930 forward For this promoter, there
Read more >
[RS-EPT-CBR] [11]
  promoter TSS_293 (cluster) 174942 forward For this promoter, there
Read more >
[RS-EPT-CBR] [11]
  promoter TSS_294 (cluster) 175008 forward For this promoter, there
Read more >
[RS-EPT-CBR] [11]
  promoter TSS_295 175012 forward nd [RS-EPT-CBR] [11]
  promoter clcAp10 175052 forward Similarity to the consensus
Read more >
[ICWHO] [12]
  promoter TSS_296 175074 forward nd [RS-EPT-CBR] [11]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Aiguo Z., Ruiwen D., Meizhi Z., 2019, Multi-enzymatic recycling of ATP and NADPH for the synthesis of 5-aminolevulinic acid using a semipermeable reaction system., Biosci Biotechnol Biochem 83(12):2213-2219

 [2] Cui Z., Jiang Z., Zhang J., Zheng H., Jiang X., Gong K., Liang Q., Wang Q., Qi Q., 2019, Stable and Efficient Biosynthesis of 5-Aminolevulinic Acid Using Plasmid-Free Escherichia coli., J Agric Food Chem 67(5):1478-1483

 [3] Eguchi Y., Okada T., Minagawa S., Oshima T., Mori H., Yamamoto K., Ishihama A., Utsumi R., 2004, Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli., J Bacteriol 186(10):3006-14

 [4] Grimm B., Bull A., Breu V., 1991, Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a cyanobacterium and Escherichia coli., Mol Gen Genet 225(1):1-10

 [5] Ikemi M., Murakami K., Hashimoto M., Murooka Y., 1992, Cloning and characterization of genes involved in the biosynthesis of delta-aminolevulinic acid in Escherichia coli., Gene 121(1):127-32

 [6] Kang Z., Wang Y., Gu P., Wang Q., Qi Q., 2011, Engineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose., Metab Eng 13(5):492-8

 [7] O'Neill GP., Chen MW., Soll D., 1989, delta-Aminolevulinic acid biosynthesis in Escherichia coli and Bacillus subtilis involves formation of glutamyl-tRNA., FEMS Microbiol Lett 51(3):255-9

 [8] Zhang J., Kang Z., Chen J., Du G., 2015, Optimization of the heme biosynthesis pathway for the production of 5-aminolevulinic acid in Escherichia coli., Sci Rep 5:8584

 [9] Zhang J., Kang Z., Ding W., Chen J., Du G., 2016, Integrated Optimization of the In Vivo Heme Biosynthesis Pathway and the In Vitro Iron Concentration for 5-Aminolevulinate Production., Appl Biochem Biotechnol 178(6):1252-62

 [10] Zhang J., Weng H., Zhou Z., Du G., Kang Z., 2019, Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli., Bioresour Technol 274:353-360

 [11] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [12] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


RegulonDB