RegulonDB

Gene
Name:	htpX
Synonym(s):	ECK1828, EG10462, b1829
Genome position(nucleotides):	1911695 <-- 1912576 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.51
External database links:

ASAP:	ABE-0006087
CGSC:	32320
ECHOBASE:	EB0457
ECOLIHUB:	htpX
OU-MICROARRAY:	b1829
STRING:	511145.b1829
COLOMBOS:	htpX

Product

Name:

protease HtpX

Synonym(s):

HtpX, zinc dependent endoprotease

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

31.923

Isoelectric point:

7.161

Motif(s):

Type	Positions	Sequence
	34 -> 54	GLMIMALLFGFGGSFVSLLMS
	73 -> 288	ERERWLVNTVATQARQAGIAMPQVAIYHAPDINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVNTFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFSRHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMALCINGKSKSLSELFMTHPPLDKRIEALRT
	4 -> 24	IALFLLTNLAVMVVFGLVLSL
	222 -> 222	E
	226 -> 226	D

Classification:

Multifun Terms (GenProtEC)
		5 - cell processes --> 5.5 - adaptations --> 5.5.2 - temperature extremes
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0008233 - peptidase activity GO:0016787 - hydrolase activity GO:0046872 - metal ion binding GO:0008270 - zinc ion binding GO:0004222 - metalloendopeptidase activity GO:0008237 - metallopeptidase activity
	biological_process	GO:0006508 - proteolysis GO:0009266 - response to temperature stimulus

Note(s):

Note(s): ...[more].

Evidence:

[APPHINH] Assay of protein purified to homogeneity from its native host
[IEP] Inferred from expression pattern

Reference(s):

[1] Arolas JL., et al., 2014
[2] Kornitzer D., et al., 1991
[3] Sakoh M., et al., 2005

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

htpX

Operon arrangement:

Transcription unit	Promoter
htpX	htpXp

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2175	1912259	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_2177	1913805	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_2178	1914229	reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Arolas JL., Garcia-Castellanos R., Goulas T., Akiyama Y., Gomis-Ruth FX., 2014, Expression and purification of integral membrane metallopeptidase HtpX., Protein Expr Purif 99C:113-118
[2] Kornitzer D., Teff D., Altuvia S., Oppenheim AB., 1991, Isolation, characterization, and sequence of an Escherichia coli heat shock gene, htpX., J Bacteriol 173(9):2944-53
[3] Sakoh M., Ito K., Akiyama Y., 2005, Proteolytic activity of HtpX, a membrane-bound and stress-controlled protease from Escherichia coli., J Biol Chem 280(39):33305-10
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.