RegulonDB RegulonDB 10.9: Gene Form
   

hycA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

hycB hycA hypA hycC FNR FNR ModE FhlA FhlA NsrR IHF FhlA FhlA anti-anti-terminator anti-terminator terminator hypBp hypBp hypAp hypAp hycAp hycAp

Gene      
Name: hycA    Texpresso search in the literature
Synonym(s): ECK2720, EG10474, b2725, hevA
Genome position(nucleotides): 2849974 <-- 2850435 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 50.22
External database links:  
ASAP:
 ABE-0008952
CGSC:
 33143
ECHOBASE:
 EB0469
ECOLIHUB:
 hycA
OU-MICROARRAY:
 b2725
STRING:
 511145.b2725
COLOMBOS: hycA


Product      
Name: regulator of the transcriptional regulator FhlA
Synonym(s): HevA, HycA
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 17.627
Isoelectric point: 6.148
Motif(s):
 
Type Positions Sequence
1 -> 146 MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGMIDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLV

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
  3 - regulation --> 3.3 - genetic unit regulated --> 3.3.1 - operon
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
Note(s): Note(s): ...[more].
Evidence: [AIFS] Automated inference of function from sequence
[IMP] Inferred from mutant phenotype
Reference(s): [1] Perez-Rueda E., et al., 2004
[2] Sauter M., et al., 1992
External database links:  
ECOCYC:
 EG10474-MONOMER
ECOLIWIKI:
 b2725
INTERPRO:
 IPR021285
PFAM:
 PF11046
PRIDE:
 P0AEV4
PRODB:
 PRO_000022954
REFSEQ:
 NP_417205
UNIPROT:
 P0AEV4


Operon      
Name: hycABCDEFGHI         
Operon arrangement:
Transcription unit        Promoter
hycABCDEFGHI


Transcriptional Regulation      
Display Regulation             
Activated by: FhlA, IHF, ModE
Repressed by: NsrR
Growth Conditions:

[1] 

C: Escherichia coli| TGYEP| nickel chloride 5 然; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| OD600 from 0.4 to 0.6
E: Escherichia coli| TGYEP| nickel chloride 5 然; sodium formate 30 mM; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| OD600 from 0.4 to 0.6

[2] 

C: Escherichia coli| fhlA knockout mutant; plasmid pSU2719 mutant| TGYEP| nickel chloride 5 然; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| OD600 from 0.4 to 0.6
E: Escherichia coli| fhlA knockout mutant; plasmid pSKOG (K. oxytoca hydG) mutant| TGYEP| nickel chloride 5 然; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| OD600 from 0.4 to 0.6

[3] 

C: Escherichia coli| TGYEP| nickel chloride 5 然; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| oxygen atom| OD600 from 0.4 to 0.6
E: Escherichia coli| TGYEP| nickel chloride 5 然; sodium formate 30 mM; sodium molybdate 1 然; sodium selenite 1 然| pH 6.5| oxygen atom| OD600 from 0.4 to 0.6


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Perez-Rueda E., Collado-Vides J., Segovia L., 2004, Phylogenetic distribution of DNA-binding transcription factors in bacteria and archaea., Comput Biol Chem 28(5-6):341-50

 [2] Sauter M., Bohm R., Bock A., 1992, Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coli., Mol Microbiol 6(11):1523-32

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