RegulonDB

Gene
Name:	hycF
Synonym(s):	ECK2715, EG10479, b2720, hevF
Genome position(nucleotides):	2844210 <-- 2844752 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.62
External database links:

ASAP:	ABE-0008942
CGSC:	33154
ECHOBASE:	EB0474
ECOLIHUB:	hycF
OU-MICROARRAY:	b2720
STRING:	511145.b2720
COLOMBOS:	hycF

Product

Name:

formate hydrogenlyase subunit HycF

Synonym(s):

HevF, HycF, formate hydrogenlyase complex, iron-sulfur protein HycF, hydrogenase 3, Fe-S subunit HycF

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,cytosol

Molecular weight:

20.309

Isoelectric point:

7.19

Motif(s):

Type	Positions	Sequence
	66 -> 95	LAWEFNLGHCIFCGRCEEVCPTAAIKLSQE
	39 -> 89	QCIGCAACVNACPSNALTVETDLATGELAWEFNLGHCIFCGRCEEVCPTAA
	31 -> 60	GKPEQNPQQCIGCAACVNACPSNALTVETD

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.5 - fermentation
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0016020 - membrane GO:0045272 - plasma membrane respiratory chain complex I
	molecular_function	GO:0046872 - metal ion binding GO:0016651 - oxidoreductase activity, acting on NAD(P)H GO:0051536 - iron-sulfur cluster binding GO:0051539 - 4 iron, 4 sulfur cluster binding
	biological_process	GO:0009060 - aerobic respiration GO:0055114 - oxidation-reduction process

Note(s):

Note(s): ...[more].

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

MINT:

MODBASE:

PANTHER:

PFAM:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMR:

SWISSMODEL:

UNIPROT:

Operon

Name:

hycABCDEFGHI

Operon arrangement:

Transcription unit	Promoter
hycABCDEFGHI	hycAp

Transcriptional Regulation
	Display Regulation
Activated by:	FhlA, IHF, ModE
Repressed by:	NsrR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3021	2843079		reverse	nd	[RS-EPT-CBR]	[1]
	promoter	hycIp4	2843102		reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.8. P-value: 9.904e-5 Read more >	[ICWHO], [RS-EPT-CBR]	[1], [2]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

Reference(s)

[1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78