RegulonDB

Gene
Name:	hypC
Synonym(s):	ECK2723, EG10485, b2728
Genome position(nucleotides):	2851864 --> 2852136
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	55.31
External database links:

ASAP:	ABE-0008962
CGSC:	33113
ECHOBASE:	EB0480
ECOLIHUB:	hypC
OU-MICROARRAY:	b2728
STRING:	511145.b2728
COLOMBOS:	hypC

Shine dalgarno
Sequence:	tgaactggctGGAGACacagcgATG

Product

Name:

hydrogenase maturation factor HypC

Synonym(s):

HypC, HypC, protein involved in hydrogenase 3 maturation

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

9.732

Isoelectric point:

3.961

Motif(s):

Type	Positions	Sequence	Comment
	1 -> 71	MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRVGQWVLVHVGFAMSVINEAEARDTLDAL
	2 -> 2	C	UniProt: Lack of hydrogenase 3 activity. Cannot interact with pre-HycE or HypD. Does not bind iron..
	6 -> 6	P	UniProt: No change in hydrogenase 3 activity. Does not affect interaction with HypD..
	51 -> 51	H	UniProt: Does not bind iron..

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.3 - energy metabolism, carbon --> 1.3.7 - anaerobic respiration
		2 - information transfer --> 2.3 - protein related --> 2.3.4 - chaperoning, repair (refolding)
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0005515 - protein binding GO:0005506 - iron ion binding GO:0042802 - identical protein binding GO:1902670 - carbon dioxide binding
	biological_process	GO:0051604 - protein maturation

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity

Reference(s):

[1] Drapal N., et al., 1998
[2] Hartwig S., et al., 2015

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PFAM:

PRIDE:

PRINTS:

PRODB:

PROSITE:

REFSEQ:

SMR:

UNIPROT:

Transcriptional Regulation
	Display Regulation
Activated by:	FhlA, FNR, IHF
Repressed by:	NsrR

Reference(s)
[1] Drapal N., Bock A., 1998, Interaction of the hydrogenase accessory protein HypC with HycE, the large subunit of Escherichia coli hydrogenase 3 during enzyme maturation., Biochemistry 37(9):2941-8
[2] Hartwig S., Thomas C., Krumova N., Quitzke V., Turkowsky D., Jehmlich N., Adrian L., Sawers RG., 2015, Heterologous complementation studies in Escherichia coli with the Hyp accessory protein machinery from Chloroflexi provide insight into [NiFe]-hydrogenase large subunit recognition by the HypC protein family., Microbiology 161(11):2204-19